Protocol II: 1 M Tris-HCl Buffer Stock Solution (1 liter)


  1. Solution A: 
    Dissolve 121.14 g Tris (American Bioanalytical #AB14042) in 800 ml dH2O.
  2. Adjust pH to 7.0 with the appropriate volume of concentrated HCl. Bring final volume to 1 liter with deionized water.
  3. Autoclave and store at room temperature.