Protocol for a Typical miRNA Methylation Reaction (M0228)

Combine the following components in sterile microfuge tube:

10X NEBuffer	2.5 µl
3.2 mM SAM	1 µl
miRNA duplex (100–500 ng	X
H2O	X
RNase inhibitor (optional)	20 units
HEN 1 RNA Methyltransferase (50 units/µl)	1 μl
Total reaction volume	25 μl

Incubate at 37°C for 1 hour.