NEBNext DNA Sample Prep Master Mix Set for 454 Quick Ligation Module Protocol (E6070)


  1. Mix the following components in a sterile microfuge tube: 
    End Repaired, Blunt or dA-Tailed DNA:  30 μl
    Quick Ligation Reaction Buffer (5X):  10 μl
    DNA Adaptors(not provided please use adaptors appropriate to specific application):  5 μl
    Quick T4 DNA Ligase:  5 μl 
    Total volume:  50 μl
  2. Incubate in a thermal cycler for 15 minutes at 20°C.
  3. Purify DNA sample on one column and elute in 25 μl of sterile dH2O or elution buffer.