NEBNext Quick Ligation Module Protocol (E6056)
- Mix the following components in a sterile microfuge tube:
End Repaired, Blunt or dA-Tailed DNA variable NEBNext Quick Ligation Reaction Buffer (5X) 10 μl 10 µM DNA Adaptors (not provided please use adaptors appropriate to specific application) variable Quick T4 DNA Ligase 5 μl Sterile H2O variable Total volume 50 μl
- Incubate in a thermocycler for 15 minutes at 20°C with heated lid set to 30°C (or off).
- Purify DNA sample using AMPure® XP or SPRIselect®® beads.
NOTE: For details on how this module is used in the NEBNext® Library Prep for Illumina® workflow, please see the manual NEBNext® DNA Library Prep Master Mix Set for Illumina® (NEB #E6040).