Starting Material: 10 ng of chromatin-immunoprecipitated (ChIP) qPCR verified or control DNA in ≤ 85 μl of water or elution buffer.
| ChIP DNA | 1–85 μl |
| NEBNext End Repair Reaction Buffer (10X) | 10 μl |
| NEBNext End Repair Enzyme Mix | 5 μl |
| Sterile H2O | variable |
| Total volume | 100 μl |
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