NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
- Starting Material: 0.5 μg of DNA Fragmented to 100–1000 bp in 16 μl of TE.
In a 1.7 ml micro-centrifuge tube add:
End Prep Enzyme Mix 1.0 μl End Repair Reaction Buffer (10X) 2.5 μl Nuclease -free Water 5.5 μl Total 9.0 μl
- Mix by pipetting and add to the 16 μl fragmented DNA sample.
- Vortex briefly to mix, followed by a quick spin to collect all liquid from the sides of the tube.
- In a thermocycler, with the heated lid on, run the following program:
20 minutes @ 25°C
20 minutes @ 72°C
Hold at 4°C