NEBNext Adaptor Ligation (E6115)

Protocol

1. After completion of the End Repair/dA-Tailing step add 1.0 μl of adaptor (or vector if generating an expression library), to the reaction tube. (Adaptors/vectors are not provided. Please use adaptors/vectors appropriate to specific application).
2. Add 1.0 μl of Quick T4 DNA Ligase. Vortex briefly to mix, followed by a quick spin.
3. Incubate for 10 minutes at 25°C.