dA-Tailing of cDNA Library (E6110)


  1. Mix the following components in a sterile 1.5 ml microcentrifuge tube:
    Purified, End Repaired cDNA: 42 μl
    10X NEBNext dA-Tailing Reaction Buffer: 5 μl
    Klenow Fragment (3´→5´ exo): 3 μl
    Total volume: 50 μl
  2. Incubate in a heat block for 30 minutes at 37°C.
  3. Purify dA-Tailed cDNA using a PCR column purification kit, purifying the sample on one column and eluting in 38 μl sterile water or elution buffer.