Capture Methylated CpG DNA (E2600)
- In a clean tube, add 5 ng-10 ug of fragmented sample DNA plus DNase free water to 65 µl.
- Add 20 µl of 5X Bind/Wash Buffer.
- Add 15 μl of well-mixed MBD2-Fc / Protein A Magnetic Beads.
- Mix by pipetting up and down.
- Incubate the reaction for 20 minutes at room temperature with rotation.