Capture and Elute Step for Control DNA (E2600)
Included in the kit are control reagents and primers to detect a methylated region, LINE element, an active gene exon, RPL30, and a micro RNA control region, MirA from a sample of fractionated HeLa DNA. (Sequences of the primers and target DNA are listed in the appendix section). A typical control reaction, that will yield a sufficient quantity of eluted material to perform qPCR reactions in triplicate, is outlined below.
- Perform capture experiment using 5 μl (0.5 ìg) of fractionated HeLa Control DNA and 10 μl MBD2-Fc beads as described above.
- Elute the beads by incubation at 65°C in 100 μl of DNase-free water.