Monarch^® gDNA Purification Columns

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
T3017L     25       Monarch® gDNA Purification Columns T3017-1 25 2 x 50 columns Not Applicable   Monarch® Collection Tubes II T2018-1 25 4 x 50 tubes Not Applicable

1. Quick Protocol for Extraction and Purification of Genomic DNA
2. Protocol for Extraction and Purification of Genomic DNA from Cells (NEB #T3010)
3. Protocol for Extraction and Purification of Genomic DNA from Blood (NEB #T3010)
4. Protocol for Extraction and Purification of Genomic DNA from Tissues (NEB #T3010)
5. Protocol for Genomic DNA Cleanup (NEB #T3010)
6. Genomic DNA Purification from Gram-positive Bacteria and Archaea (NEB #T3010)
7. Genomic DNA Purification from Yeast (NEB #T3010)
8. Genomic DNA Purification from Saliva (NEB #T3010)
9. Genomic DNA Purification from Buccal Swabs (NEB #T3010)
10. Genomic DNA Purification from Gram-negative Bacteria (NEB #T3010)
11. Download Quick Protocol Card T3010
12. Genomic DNA Purification from Insects

• Choosing Input Amounts for the Monarch Genomic DNA Purification Kit
• Factors Affecting DNA Quality when Purifying gDNA from Blood and Tissues with the Monarch Genomic DNA Purification Kit
• Guidelines for Handling Tissue Samples when using the Monarch Genomic DNA Purification Kit

• A Practical Guide to Analyzing Nucleic Acid Concentration and Purity with Microvolume Spectrophotometers

1. What is the maximum binding capacity of the Monarch^® Genomic DNA Purification Kit columns?
2. What is the minimum elution volume that can be used with the Monarch^® Genomic DNA Purification Kit columns?
3. How important is it to pre-heat the elution buffer to 60°C? Would 56°C be OK?
4. Can I get better recovery with the Monarch® Genomic DNA Purification Kit if I do a second elution with my eluent from the first elution?
5. Can I use a different elution buffer than the one supplied with the Monarch Genomic DNA Purification Kit (NEB #T3010)?
6. What size gDNA can be purified with the Monarch^® Genomic DNA Purification Kit?
   
    
7. What is the minimal input amount that can be used with the Monarch^® Genomic DNA Purification Kit?
8. Can I use the Monarch^® Genomic DNA Purification Kit to clean up gDNA that was extracted with phenol/chloroform?
9. Can I use the Monarch^® Genomic DNA Purification Kit for gDNA cleanup?
   
    
10. How can I assess gDNA integrity and purity?
11. Can I omit the low-speed binding centrifugation step to save time?
12. Can I omit the RNase digestion step from the protocol? 
13. Is Monarch-purified gDNA suitable for long-range PCR?
14. When working with other commercial silica column-based kits, I occasionally see a white precipitate in the eluate. What is this?
15. Is Monarch-purified gDNA compatible with Next Generation Sequencing (NGS) platforms like PacBio and nanopore?
16. Why did the silica membrane on my Monarch Genomic DNA Column (NEB #T3017) dislodge during my genomic DNA prep?
17. Can the Monarch Genomic DNA Purification Kit (NEB #T3010) be used to isolate gDNA from drosophila and other insects?
18. Can the Monarch Genomic DNA Purification Kit (NEB #T3010) be used to isolate gDNA from plants?
19. Can the Monarch Genomic DNA Purification Kit (NEB #T3010) be used to isolate gDNA from plasma, serum and urine?
20. Can the Monarch Genomic DNA Purification Kit (NEB #T3010) be used for isolating cfDNA?
21. Can the Monarch Genomic DNA Purification Kit (NEB #T3010) be used with a vacuum manifold?
    

• Troubleshooting Guide for Genomic DNA Extraction & Purification (NEB #T3010)

• Monarch Nucleic Acid Purification Brochure

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Change Notifications

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• T3017L_v1
• T3017S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• T3017L_v1_10028547
• T3017L_v1_10041709
• T3017L_v1_10068618
• T3017L_v1_10104311
• T3017-1_v0_10086482
• T3017L_v1_10148339
• T3017L_v1_10144604
• T3017L_v2_10163996
• T3017L_v2_10182199

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.