NEBNext^® Ultra™ II kits are available for directional (strand-specific) or non-directional RNA library preparation. These kits utilize streamlined workflows and novel master mixes, and have been designed for performance with input amounts as low as 5 ng Total RNA.

Modules, including for rRNA Depletion and poly(A) mRNA enrichment, offer the user the ability to customize sample preparation. Adaptors and primers (NEBNext Oligos) are supplied separately, for maximum flexibility.

For microRNAs and other small RNAs, our novel Small RNA workflow has been optimized to minimize adaptor-dimers, while producing high-yield, high-density libraries.

• The NEBNext^® Ultra™ II Directional RNA Library Prep Kit for Illumina^® (E7760S/L) uses the dUTP method and a streamlined workflow to generate high yield, high quality strand-specific libraries.
• The NEBNext^® Ultra™ II RNA Library Prep Kit for Illumina^® (E7770S/L) enables production of non-directional libraries using a streamlined workflow.