NEBNext2_Ban_668x162

RNA Library Prep

NEBNext® Ultra™ II kits are available for directional (strand-specific) or non-directional RNA library preparation. These kits utilize streamlined workflows and novel master mixes, and have been designed for performance with input amounts as low as 5 ng Total RNA.

Modules, including for rRNA Depletion and poly(A) mRNA enrichment, offer the user the ability to customize sample preparation. Adaptors and primers (NEBNext Oligos) are supplied separately, for maximum flexibility.

For microRNAs and other small RNAs, our novel Small RNA workflow has been optimized to minimize adaptor-dimers, while producing high-yield, high-density libraries.

  • The NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (E7760S/L) uses the dUTP method and a streamlined workflow to generate high yield, high quality strand-specific libraries.
  • The NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (E7770S/L) enables production of non-directional libraries using a streamlined workflow.

  1. Tips for Optimizing RNA Inputs in NGS Library Construction

    Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.

  2. A Breakthrough Method of Small RNA Sample Preparation

    The common problem of adaptor dimer formation during small RNA library construction can be avoided by using NEBNext® protocols. Learn about this technique, and how it improves both performance and sensitivity in library construction.

  3. Tips for Bead-based Clean ups and Size Selection

    Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

  4. 12 Quick Tips for NGS Library Preparation

    Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

  5. NEBNext® Ultra™ II Directional RNA Workflow

    Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.





Featured Products

Other Tools and Resources

Ultra II Directional RNA Library Preparation Workflow for Illumina

Workflow"

Ultra II  Non-directional RNA Library Preparation Workflow for Illumina

Workflow"

Small RNA Library Preparation Workflow for Illumina

Workflow"