NEBNext2_Ban_668x162

Microbiome DNA Enrichment

Microbiome DNA analysis can be challenging due to the high percentage of host DNA present in many samples. The NEBNext® Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment. The NEBNext Microbiome DNA Enrichment Kit is suitable for a wide range of sample types and is compatible with downstream applications including next generation sequencing on all platforms, qPCR and end-point PCR.

  1. Behind the Paper: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues

    Can microbiome-enrichment techniques be used to partition plant DNA? Erbay describes the findings of his recent paper.

  2. NEBNext Ultra II DNA Library Prep Protocol

    This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.

  3. Library prep for NGS

    Get a quick overview of NGS library preparation from an NEB scientist.

  4. Tips for Optimizing DNA Inputs in NGS Library Construction

    Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips.

  5. 12 Quick Tips for NGS Library Preparation

    Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.




Featured Products

    Publications related to Microbiome DNA Enrichment:

  1. Erbay Yigit, David I. Hernandez, Joshua T. Trujillo, Eileen Dimalanta, and C. Donovan Bailey (2014). Genome and Metagenome Sequencing: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues. Applications in Plant Sciences. 2 (11), 1400064. PubMedID: 25383266, DOI: 10.3732/apps.1400064
  2. George R. Feehery, Erbay Yigit, Samuel O. Oyola, Bradley W. Langhorst, Victor T. Schmidt, Fiona J. Stewart, Eileen T. Dimalanta, Linda A. Amaral-Zettler, Theodore Davis, Michael A. Quail, Sriharsa Pradhan (2013). A Method for Selectively Enriching Microbial DNA from Contaminating Vertebrate Host DNA PLOS ONE. 8 (10), e76096. PubMedID: 24204593

Other Tools and Resources

Microbiome DNA Enrichment Workflow

workflow