Microbiome DNA Enrichment

Microbiome DNA analysis can be challenging due to the high percentage of host DNA present in many samples. The NEBNext® Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment. The NEBNext Microbiome DNA Enrichment Kit is suitable for a wide range of sample types and is compatible with downstream applications including next generation sequencing on all platforms, qPCR and end-point PCR.
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FAQs for Microbiome DNA Enrichment
Protocols for Microbiome DNA Enrichment
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    Feature Articles

  • Addressing Challenges in Microbiome DNA Analysis

    Microbiome sample analysis is commonly confounded by the presence of host-cell DNA in the sample. The NEBNext® Microbiome DNA Enrichment Kit enables enrichment of non-CpG-methylated DNA from samples contaminated with eukaryotic-cell DNA.

  • Brochures

  • NEBNext for Ion Torrent™ Brochure

    The NEBNext Brochure for Ion Torrent provides an overview of library preparation for DNA for the Ion Torrent platform, as well as useful technical tips.

    Publications related to Microbiome DNA Enrichment
  1. Erbay Yigit, David I. Hernandez, Joshua T. Trujillo, Eileen Dimalanta, and C. Donovan Bailey 2014. Genome and Metagenome Sequencing: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues. Applications in Plant Sciences. 2 (11), PubMedID: 25383266, DOI: 10.3732/apps.1400064
  2. George R. Feehery, Erbay Yigit, Samuel O. Oyola, Bradley W. Langhorst, Victor T. Schmidt, Fiona J. Stewart, Eileen T. Dimalanta, Linda A. Amaral-Zettler, Theodore Davis, Michael A. Quail, Sriharsa Pradhan 2013. A Method for Selectively Enriching Microbial DNA from Contaminating Vertebrate Host DNA PLOS ONE. 8 (10), PubMedID: 24204593, DOI:
Posters and Manuals
Microbiome DNA Enrichment Workflow

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Legal Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.