Microbiome DNA Enrichment

Microbiome DNA analysis can be challenging due to the high percentage of host DNA present in many samples. The NEBNext® Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment. The NEBNext Microbiome DNA Enrichment Kit is suitable for a wide range of sample types and is compatible with downstream applications including next generation sequencing on all platforms, qPCR and end-point PCR.
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FAQs for Microbiome DNA Enrichment

Protocols for Microbiome DNA Enrichment

    Publications related to Microbiome DNA Enrichment

  1. George R. Feehery, Erbay Yigit, Samuel O. Oyola, Bradley W. Langhorst, Victor T. Schmidt, Fiona J. Stewart, Eileen T. Dimalanta, Linda A. Amaral-Zettler, Theodore Davis, Michael A. Quail, Sriharsa Pradhan 2013. A Method for Selectively Enriching Microbial DNA from Contaminating Vertebrate Host DNA PLOS ONE. 8 (10), PubMedID: 24204593, DOI:
  2. Erbay Yigit, David I. Hernandez, Joshua T. Trujillo, Eileen Dimalanta, and C. Donovan Bailey 2014. Genome and Metagenome Sequencing: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues. Applications in Plant Sciences. 2 (11), PubMedID: 25383266, DOI: 10.3732/apps.1400064

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Microbiome DNA Enrichment Workflow

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