qPCR is considered to be the most accurate and effective method of library quantitation, providing considerably higher consistency and reproducibility of quantitation. The qPCR-based NEBNext® Library Quant Kit quantitates only those molecules that contain both adaptor sequences, thereby providing a more accurate estimate of the concentration of the library molecules that can be sequenced.
FAQs for Library Quantitation
Protocols for Library Quantitation
NEBNext for Ion Torrent Brochure
The NEBNext Brochure for Ion Torrent provides an overview of library preparation for DNA for the Ion Torrent platform, as well as useful technical tips.
NEBNext® for Illumina® Brochure
The NEBNext Brochure for Illumina provides an overview of library preparation for DNA, ChIP-Seq, RNA and Small RNA for the Illumina platform, as well as tech tips and select peer-reviewed citations.
The Quantitation Question: How does accurate library quantitation influence sequencing?
The determination of the number of sequencing-ready molecules present after library preparation is an important step in the next generation sequencing (NGS) workflow and has a strong influence on the success of both a sequencing run and a sequencing-based experiment.
Other Tools & Resources
NEBNext Library Quant Kit Workflow
Posters and Manuals
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.