Library Quantitation

The determination of the number of sequencing-ready molecules present after library preparation is an important step in the next generation sequencing (NGS) workflow and has a strong influence on the success of a sequencing run.

qPCR is considered to be the most accurate and effective method of library quantitation, providing considerably higher consistency and reproducibility of quantitation. The qPCR-based NEBNext® Library Quant Kit quantitates only those molecules that contain both adaptor sequences, thereby providing a more accurate estimate of the concentration of the library molecules that can be sequenced.

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FAQs for Library Quantitation

Protocols for Library Quantitation

    Other Tools & Resources

    Brochures

  • NEBNext for Ion Torrent Brochure

    The NEBNext Brochure for Ion Torrent provides an overview of library preparation for DNA for the Ion Torrent platform, as well as useful technical tips.

  • NEBNext® for Illumina® Brochure

    The NEBNext Brochure for Illumina provides an overview of library preparation for DNA, ChIP-Seq, RNA and Small RNA for the Illumina platform, as well as tech tips and select peer-reviewed citations.

  • Feature Articles

  • The Quantitation Question: How does accurate library quantitation influence sequencing?

    The determination of the number of sequencing-ready molecules present after library preparation is an important step in the next generation sequencing (NGS) workflow and has a strong influence on the success of both a sequencing run and a sequencing-based experiment.

NEBNext Library Quant Kit Workflow

Workflow"

Posters and Manuals

Legal Information

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For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.