ngs banner

DNA Library Prep

NEBNext® reagents are available for sample preparation of genomic DNA, ChIP DNA, and FFPE DNA. These products are provided in user-friendly formats including kits and modules, with bulk and customized formats also available. Adaptors and primers are available separately, for maximum flexibility. These robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

NEBNext Ultra™ II (E7645S/L) enables the construction of high yield, high quality libraries from low picogram to microgram input amounts. A streamlined workflow offers reduced hands-on time and automation compatibility, while maintaining uniform sequencing of targets regardless of GC content. 

nebnext selector inline banner

FAQs for DNA Library Prep

Protocols for DNA Library Prep

Posters and Manuals

Posters Manuals
  • Manual for NEB #E7645

Ultra II DNA Library Preparation Workflow for Illumina

Ultra DNA Library Preparation Workflow for Illumina

DNA Library Prep Workflow for Ion Torrent

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

  1. NEBNext_UltraII_Protocol_282x210

    NEBNext Ultra II DNA Library Prep Protocol

    This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.

  2. Library Preperation For NGS

    Library prep for NGS

    Get a quick overview of NGS library preparation from an NEB scientist.

  3. NEBNextVideo_OptimizingDNAInput_thumb

    Tips for Optimizing DNA Inputs in NGS Library Construction

    Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips.

  4. NEBNextVideo_12Tips_thumb

    12 Quick Tips for NGS Library Preparation

    These 12 quick tips for NGS library preparation are a great refresher if you're a seasoned pro, or a great introduction if you're a beginner.

  5. Size Selection and Cleanup with NEBNext Ultra

    Size Selection and Cleanup with NEBNext Ultra II and SPRI beads

    This video walks you through the size selection and cleanup steps using the NEBNext Ultra II DNA