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DNA Library Prep

NEBNext® reagents are available for sample preparation of genomic DNA, ChIP DNA, and FFPE DNA. These products are provided in user-friendly formats including kits and modules, with bulk and customized formats also available. Adaptors and primers are available separately, for maximum flexibility. These robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

NEBNext Ultra™ II (E7645S/L) enables the construction of high yield, high quality libraries from low picogram to microgram input amounts. A streamlined workflow offers reduced hands-on time and automation compatibility, while maintaining uniform sequencing of targets regardless of GC content. 

  1. NEBNext Ultra II DNA Library Prep Protocol

    This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.

  2. Library prep for NGS

    Get a quick overview of NGS library preparation from an NEB scientist.

  3. Tips for Optimizing DNA Inputs in NGS Library Construction

    Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips.

  4. 12 Quick Tips for NGS Library Preparation

    Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

  5. Size Selection and Cleanup with NEBNext Ultra II and SPRI beads

    This video walks you through the size selection and cleanup steps using the NEBNext Ultra II DNA



Featured Products

Other Tools and Resources

Ultra II DNA Library Preparation Workflow for Illumina

Ultra DNA Library Preparation Workflow for Illumina

DNA Library Prep Workflow for Ion Torrent

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