- This product was discontinued on January 01, 2013
CBG-NH2 is an amine-terminated building block particularly suited for the one-step modification of carboxylic ester modified surfaces such as Biacore® CM5 sensor chips. This building block enables you to covalently modify a surface of your choice with SNAP-tag® substrate allowing directed and covalent immobilization of SNAP-tag fusion proteins.
The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.
There are two steps to using this system: cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of fusion proteins with the SNAP-tag substrate is described in this document.
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