SNAP-Surface® 782

  • Catalog # S9142 was discontinued on October 03, 2014
  • Product Information
    SNAP-Surface® 782 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This cell impermeable substrate (BG-782) is based on the Dynomics dye Dy-782 and is suitable for 780 nm laser excitation. It has an excitation maximum at 784 nm and emission maxima at 789 nm. This package includes 50 nmol of SNAP-Surface 782 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution. 

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA alkyltransferase (AGT).SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. 

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

    Figure 1:
    Excitation (dotted line) and emission spectra of SNAP-Surface 782 coupled to SNAP-tag in buffer at pH 7.5.

    Figure 2: Structure of SNAP-Surface 782 (MW 1131.31 g/mol)
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