SNAP-Surface® 682

  • Catalog # S9139 was discontinued on October 03, 2014
  • Product Information
    SNAP-Surface® 682 is a photostable fluorescent substrate that can be used to label SNAP-Tag® fusion proteins in solution. This substrate (BG-682) is based on the Dyomics dye DY-682 and is suitable for excitation with a 680 nm diode laser. It has an excitation maximum at 693 nm and emission maxima at 708 nm. This package includes 50 nmol of SNAP-Surface 682 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines, In the labeling reaction, the substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.

    There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

    Figure 1:
    Excitation (dotted line) and emission spectra of SNAP-Surface 682 coupled to SNAP-tag in buffer at pH 7.5
    Figure 2: Structure of SNAP-Surface 682 (MW 1105.22)
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