SNAP-Surface® 425

  • This product was discontinued on January 01, 2013

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S9126
  • Discontinued
    Discontinued
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    SNAP-Surface® 425 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins on cell surfaces or in solution. This substrate (BG-425) is based on the ATTO-TEC dye ATTO 425 and is suitable for appropriate blue lasers and filter sets. It has an excitation maximum at 438 nm and emission maximum at 489 nm. This package includes 50 nmol of SNAP-Surface 425 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

    Figure 1:
    Excitation (dotted line) and emission spectra of SNAP-Surface 425 coupled to SNAP-tag in buffer at pH 7.5
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    Figure 2:  Structure of SNAP-Surface 425 (MW 725.8 g/mol)
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