SNAP-Cell® Fluorescein

SNAP-Cell® Fluorescein is a green fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells or in vitro.  This substrate has limited photostability.

  • It is a cell-permeable substrate (BG-Fluorescein) based on diacetylfluorescein
  • Diacetylfuorescein is essentially non-fluorescent, but it becomes fluorescent inside the cell when it is hydrolyzed by non-specific esterases, yielding fluorescein
  • It is suitable for standard fluorescein filter sets
  • It has an excitation maximum at 500 nm and an emission maximum at 532 nm

Ordering Information

$name
  • 50 nmol
    $307.00
  • Product Information
    This package contains 50 nmol of SNAP-Cell Fluorescein substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution. This substrate has limited photostability. If this presents a problem, we recommend using SNAP-Cell 505, which has similar spectral characteristics but much greater photostability. 

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines, In the labeling reaction, the substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of fusion proteins with the SNAP-tag substrate is described below.

    Figure 1: Live CHO-K1 cells transiently transfected with pSNAP-H2B

    Figure 1: Live CHO-K1 cells transiently transfected with pSNAP-H2B
    Cells were labeled with SNAP-Cell Fluorescein (green) for 15 minutes.
    Figure 2:  Excitation (dotted line) and emission spectra of SNAP-Cell Fluorescein (fluorescent product of SNAP-Cell Fluorescein in cells) coupled to SNAP-tag in buffer at pH 7.5)Figure 2: Excitation (dotted line) and emission spectra of SNAP-Cell Fluorescein (fluorescent product of SNAP-Cell Fluorescein in cells) coupled to SNAP-tag in buffer at pH 7.5)


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    Figure 3:  Structure of SNAP-Cell Fluorescein (MW 712.7g/mol)
    Product Categories:
    SNAP-tag Substrates,
    Protein Labeling Products
    Applications:
    In vivo Imaging,
    Protein Localization,
    Cell Imaging
    ,
    Protein Labeling (SNAP/CLIP)
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