polyA Spin™ mRNA Isolation Kit

We recommend Oligo d(T)25 Magnetic Beads (NEB #S1419S) as an alternative for purification of mRNA containing polyadenylic (poly A) regions. For NGS applications, the NEBNext Poly(A) mRNA Isolation Module (NEB #E7490) is recommended.
  • Catalog # S1560 was discontinued on March 31, 2020 ;

This kit contains an affinity matrix used for the isolation of mRNA containing polyadenylic (poly A) regions (1) from purified total RNA. This matrix consists of oligo (dT)25 covalently coupled to a cross-linked cellulose bead. This product is guaranteed for twenty-four months when stored at 4°C in the supplied storage buffer.

  • A rapid and convenient alternative to traditional column chromatography for the isolation of full-length poly(A)+ eukaryotic messenger (mRNA) from samples of Total RNA
  • Shorter purification time: Prepare full-length mRNA in as little as 40 minutes
  • Maintains Resolution: When compared to a 100 mg column of high quality oligo (dT) cellulose in 1.0 mg total RNA sample range
Learn more about how this product is being used in the Product Citation Tool
  • Product Information
    The polyA Spin™ Kit is a rapid and convenient alternative to traditional column chromatography for the isolation of full-length poly(A)+ eukaryotic messenger (mRNA) from samples of "total" RNA. Poly(A)+ RNA selection is made by affinity chromatography using spin columns prepackaged with NEB's Oligo (dT)25-Cellulose beads (NEB #S1408S). Prepared essentially by the method of Gilham (1), this solid support has found widespread use in the "batch" preparation of low abundance mRNA (2). Its high binding capacity and rapid hybridization kinetics make it an ideal support for chromatographic poly(A)+ RNA selection. Intact mRNA can be isolated in as little as forty minutes from multiple cell lysates or samples of "total" RNA by spin chromatography in a microcentrifuge.
    Reagents sufficient for the isolation and subsequent precipitation of poly(A)+ RNA from eight samples of as much as 1.0 mg of "total" RNA are provided. The isolated RNA can be used for in vitro translation experiments, the preparation of cDNA libraries, Northern analysis, subtractive hybridization or differential display.

    Kit Components

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    5 M NaCl 500 mM
    3M NaAC 300 mM
    Wash Buffer I 1 X
    Wash Buffer (15 ml) 1 X
    Elution Buffer 1 X
    Low Salt Buffer 1 X
    Glycogen Solution 10 mg/ml
    microcentrifuge tubes containing oligo (dT)25 -cellulose beads
    microcentrifuge spin columns
    sterile microcentrifuge tubes
    Product Categories:
    Discontinued Products
    • Advantages and Features
    • Properties & Usage
    • Product Notes
    • References
  • Protocols, Manuals & Usage
  • FAQs & Troubleshooting
    • Tech Tips
  • Citations & Technical Literature
    • Citations
  • Quality, Safety & Legal
    • Quality Control Assays
    • Specifications
    • Certificate of Analysis
    • Safety Data Sheets
    • Legal and Disclaimers
Loading Spinner