polyA Spin™ mRNA Isolation Kit

Features

• Shorter Purification Time
• Reduced Isolation Volumes
• Maintains Resolution: When compared to a 100 mg column of high quality oligo (dT) cellulose in 1.0 mg total RNA sample range.

1. The polyA Spin mRNA Isolation Kit complements and serves as an alternative to traditional column chromatography isolation of mRNA. Each polyA Spin spin column has the same column performance as a standard 100 mg column of high quality oligo (dT)-cellulose in the 1.0 mg total RNA sample range. Thus the shorter purification times and reduced working volumes associated with spin chromatography are realized while maintaining column chromatography levels of performance. This is of particular importance in the isolation of low abundance messenger RNA (1). For total RNA samples greater than 1.0 mg initial "first round" isolation of poly(A)+ material should be done by column or "batch" (NEB #S1408S) chromatography (2). First round isolation of poly(A)+ RNA by either column or spin chromatography typically yields a product contaminated with poly(A)– RNA. PolyA Spin spin chromatography is ideal for "second round" purification, yielding material with an A260/280 of 1.9 or greater with minimal product loss. The protocol has been optimized for the isolation of poly(A)+ RNA from 0.1–1.0 mg of total RNA isolated from eukaryotic tissue or cells that contain between 1–5% mRNA. The amount of poly(A)+ RNA isolated will vary with the type of tissue or cells used.
2. This product is not designed to work with in-vitro RNA.

1. Gilham, P.T. (1964). J. Amer. Chem. Soc.. 86, 4982.
2. Farrell, R.E. (1993). RNA Methodologies. 104-106.
3. Jacobson, A. (1987). S. Berger and A. Kimmel(Ed.), Methods Enzymol.. 152, 254-257. San Diego: Academic Press.

1. Isolation of mRNA Using the PolyA Spin™ mRNA Isolation Kit
2. Batch Method Protocol (S1560) for Isolation of mRNA Using the PolyA Spin™ mRNA Isolation Kit

• cDNA/Reverse Transcriptase Tips

Product Citation Tool

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• S1560S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• S1560S_v0_10009227
• S1560S_v0_10020511
• S1560S_v1_10033588
• S1560S_v1_10046561
• S1560S_v1_10051851

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