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  • RNA Synthesis Kits

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    Learn about RNA Synthesis

    HiScribe™ T7 High Yield RNA Synthesis Kit

    The T7 High Yield RNA Synthesis Kit (NEB #E2040) is an extremely flexible system for in vitro transcription of RNA using T7 RNA polymerase. The kit allows for synthesis of many kinds of RNA including internally labeled and co-transcriptionally capped transcripts. RNA synthesized from the kit is suitable for many applications including RNA structure and function studies, ribozyme biochemistry, probes for RNase protection assays and hybridization-based blots, anti-sense RNA and RNAi experiments, microarray analysis, microinjection and in vitro translation and RNA vaccines.

    Advantages of the HiScribe™ T7 High Yield RNA Synthesis Kit

    • Streamlined format
    • Flexibility for high yield production of long and short RNA (up to 180 μg of RNA from 1 μg of control template)
    • Shelf life is 1 year or longer

    HiScribe™ T7 Quick High Yield RNA Synthesis Kit

    The HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB #E2050) is designed for quick set-up and production of large amounts of RNA in vitro. The reaction can be set up conveniently by combining the NTP buffer mix, T7 RNA Polymerase mix and a suitable DNA template. The kit also allows for capped RNA or dye-labeled RNA synthesis by incorporation of cap analog (ARCA, NEB #S1411) or dye-modified nucleotides. RNA synthesized with the kit can be used for RNA structure and function studies, ribozyme biochemistry, as probes for RNase protection assays and hybridization based blots, anti-sense RNA and RNAi experiments, microarray analysis and microinjection, as well as in vitro translation and RNA vaccines.

    Advantages of the HiScribe™ T7 Quick High Yield RNA Synthesis Kit

    • Quicker setup
    • Enables synthesis of large amounts of RNA
    • Shelf life is 1 year or longer

    HiScribe™ is a trademark of New England Biolabs, Inc.