T7 (NEB #M0251) and SP6 (NEB #M0207)
RNA polymerases are DNA dependent RNA polymerases that produce DNA
templated RNA transcripts. T7 and SP6 exhibit high specificity for their
respective promoters. Both T7 and SP6 can be used for the in vitro
synthesis of RNA for a wide variety of applications, including
transfection, translation, structural studies and radioactive and
non-isotopic probe generation. E. coli Poly(A) Polymerase (NEB #M0276) and Poly(U) Polymerase (NEB #M0337) generate untemplated homoribopolymeric tails on the 3´-ends of RNA. Both E. coli Poly(A) Polymerase and Poly(U) Polymerase can be used for RNA tailing for reverse transcription or labeling.
RNA synthesis requires a DNA template, RNA polymerase, NTPs and other
factors. High-yield robust reactions require optimization of each
reaction component. NEB offers five in vitro RNA synthesis kits,
all of which have been rigorously formulated to provide reproducible
high yields of quality RNA. Additionally, NEB offers a Vaccinia capping
system (NEB #M2080) and a number of RNA cap analogs.
Learn more about the HiScribe in vitro transcription kits.