RNA Modification
New England Biolabs has an extensive offering of enzymes that modify RNA. RNA modifying enzymes are used to prepare RNA for cloning (NEB #M0290, NEB #M0201, NEB #M0356), for direct RNA labeling (NEB #M0201, NEB #M0228), and for RNA modification before transfection (NEB #M2080, NEB #M0366), among numerous other applications.
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Protocols for RNA Modification
- Decapping eukaryotic mRNA with RppH (NEB #M0356)
- A Typical Deadenylation Reaction (M0331)
- Protocol for Dephosphorylation of 5´-ends of DNA using rSAP (M0371)
- Protocol for Dephosphorylation of 5´-ends of DNA using Quick Dephosphorlyation Kit (M0508)
- Protocol for Dephosphorylation of 5'-ends of DNA using CIP (NEB #M0290)
- Non-radioactive Phosphorylation with T4 PNK or T4 PNK (3´ phosphatase minus)
- Protocol for Dephosphorylation of 5´-ends of DNA using Antarctic Phosphatase (NEB #M0289)
- Capping Protocol (M2080)
- Radioactive Labeling with T4 PNK or T4 PNK (3´ phosphatase minus)
- Labeling Protocol (M2080)
- One-Step Capping and 2´-O-Methylation (M0366)
- 2´-O-Methylation of Capped RNA (M0366)
- Enzymatic PCR Cleanup Protocol
- Ligation of a DNA or RNA oligo to single stranded RNA using T4 RNA Ligase 1
- Ligation of a DNA or RNA oligo to single stranded RNA using T4 RNA Ligase 1
- A Typical Tailing Reaction (M0337)
- End-labeling Protocol
- Poly(A) Tailing of RNA using E. coli Poly(A) Polymerase (NEB# M0276)
- Avoiding Ribonuclease Contamination
Tools & Resources
Usage Guidelines
Reagents for RNA Sample Preparation
RNA Polymerase Selection Chart
RNA Ligase Selection Chart
cDNA Synthesis Selection Chart
Legal Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
