RNA Modification
New England Biolabs has an extensive offering of enzymes that modify RNA. RNA modifying enzymes are used to prepare RNA for cloning (NEB #M0290, NEB #M0201, NEB #M0356), for direct RNA labeling (NEB #M0201, NEB #M0228), and for RNA modification before transfection (NEB #M2080, NEB #M0366), among numerous other applications.
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Protocols for RNA Modification
- Decapping eukaryotic mRNA with RppH (NEB #M0356)
- A Typical Deadenylation Reaction (M0331)
- Protocol for Dephosphorylation of 5´-ends of DNA using rSAP (M0371)
- Protocol for Dephosphorylation of 5´-ends of DNA using Quick Dephosphorlyation Kit (M0508)
- Protocol for Dephosphorylation of 5'-ends of DNA using CIP (NEB #M0290)
- Non-radioactive Phosphorylation with T4 PNK or T4 PNK (3´ phosphatase minus)
- Protocol for Dephosphorylation of 5´-ends of DNA using Antarctic Phosphatase (NEB #M0289)
- Capping Protocol (M2080)
- Radioactive Labeling with T4 PNK or T4 PNK (3´ phosphatase minus)
- Labeling Protocol (M2080)
- 2´-O-Methylation of Capped RNA (M0366)
- Enzymatic PCR Cleanup Protocol
- One-Step Capping and 2´-O-Methylation (M0366)
- Ligation of a DNA or RNA oligo to single stranded RNA using T4 RNA Ligase 1
- Ligation of a DNA or RNA oligo to single stranded RNA using T4 RNA Ligase 1
- A Typical Tailing Reaction (M0337)
- End-labeling Protocol
- Poly(A) Tailing of RNA using E. coli Poly(A) Polymerase (NEB# M0276)
- Avoiding Ribonuclease Contamination
Other Tools & Resources
Usage Guidelines
Reagents for RNA Sample Preparation
RNA Polymerase Selection Chart
RNA Ligase Selection Chart
cDNA Synthesis Selection Chart
