NEB offers a selection of products for the ligation of single-stranded or double-stranded RNA to either RNA or DNA. Our RNA Ligase Selection Chart lists all of the ligases available for RNA, along with their recommended application.
- Protocol for ligation of the 3´ OH of RNA to the 5´ pre-adenylated DNA with T4 RNA Ligase 2 truncated K227Q (NEB #M0351)
- Protocol for Oligonucleotide Adenylation (E2610)
- RNA Circularization using T4 RNA Ligase 1
- Protocol for ssDNA/RNA Ligation (M0319)
- Ligation of a DNA or RNA oligo to single stranded RNA using T4 RNA Ligase 1
- 3´ End Labeling of RNA using T4 RNA Ligase 1
- Typical Protocol for RNA Ligation (M0458)
- Avoiding Ribonuclease Contamination
- Ligate a pre-adenylated DNA or RNA sequence tag to the 3´-end of any RNA
- Join a single stranded adenylated primer to small RNAs for cDNA library creation
- Join a single stranded adenylated primer to RNA for directional cDNA library construction
- Enzymatic 5´-adenylation of ss-DNA linkers for next gen sequencing
- One step reaction gives quantitative adenylation.
- Simpler than existing chemical and enzymatic methods.
- Reduces need for purification of reaction product
- 65°C reaction temperature reduces secondary structural concerns
- Easily scalable from pmol to μmol range
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