RNA_Product_Hero

RNA Isolation & Enrichment

Studies that require the isolation of eukaryotic mRNA often take advantage of the polyadenine tail typically present at the 3’ end. One approach involves the use affinity matrices; poly-T oligonucleotides that hybridize to the complementary poly-A tails (1). Enrichment of miRNA and siRNAs can also be achieved using high-affinity binding proteins and the use of chitin magnetic beads (2, 3).

References:

  1. Aviv, H. and Leder, P. (1972) Proc. Natl. Acad. Sci. USA, 69, 1408. PMID: 4504350
  2. Silhavy, D. et al. (2002) EMBO J., 21, 3070-3080. PMID: 12065420
  3. Jin, J. et al. (2010) Biotechneques. 48, XVII-XXII. PMID: 20569217

Choose Type:

Protocols for RNA Isolation & Enrichment

RNA Ligase Selection Chart

RNA_Ligases_Selection_thumb
NEB offers a variety of ligases for RNA research with unique specificities.

cDNA Synthesis Selection Chart

cDNA_Synthesis_Selection_thumb
NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.