RNA_Product_Hero

RNA Isolation & Enrichment

Studies that require the isolation of eukaryotic mRNA often take advantage of the polyadenine tail typically present at the 3’ end. One approach involves the use affinity matrices; poly-T oligonucleotides that hybridize to the complementary poly-A tails (1). Enrichment of miRNA and siRNAs can also be achieved using high-affinity binding proteins and the use of chitin magnetic beads (2, 3).

References:

  1. Aviv, H. and Leder, P. (1972) Proc. Natl. Acad. Sci. USA, 69, 1408. PMID: 4504350
  2. Silhavy, D. et al. (2002) EMBO J., 21, 3070-3080. PMID: 12065420
  3. Jin, J. et al. (2010) Biotechneques. 48, XVII-XXII. PMID: 20569217

Choose Type:

Protocols for RNA Isolation & Enrichment

RNA Ligase Selection Chart

NEB offers a variety of ligases for RNA research with unique specificities.

cDNA Synthesis Selection Chart

NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.