Isolating high-quality RNA molecules is crucial to many downstream experiments, such as cloning,reverse transcription for cDNA library creation, sequencing and structural analysis. Studies that require the isolation of eukaryotic mRNA often take advantage of the polyadenine tail typically present at the 3’ end. One approach involves the use affinity matrices; poly-T oligonucleotides that hybridize to the complementary poly-A tails (1). Enrichment of miRNA and siRNAs can also be achieved using high-affinity binding proteins and the use of chitin magnetic beads (2, 3).
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