
Reverse Transcriptases & RT-PCR
Choose Type:
- First Strand cDNA Synthesis Protocols (E6300)
- Protocol for NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module (E6111)
- First strand cDNA synthesis OneTaq® RT-PCR Kit
- PCR Amplification with OneTaq® RT-PCR Kit
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0277)
- First Strand cDNA Synthesis Protocols (E6560)
- Loop-mediated Isothermal Amplification (LAMP)
- One-Step RT-PCR Protocols (E5315)
- Reaction Conditions (E5315)
- Standard PCR Protocol (E5315)
- Typical cDNA Synthesis Protocol with WarmStart RTx (NEB #M0380)
- Typical RT-LAMP Protocol
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0277)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0277)
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0253)
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0368)
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0253)
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0368)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0253)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0368)
- Avoiding Ribonuclease Contamination
Usage Guidelines
- Wulf, M.G., Maguire, S., Humbert, P., Dai, N., Bei, Y., Nichols, N.M., Correa, I.R., Jr., Guan, S (2019) Non templated addition and template switching by Moloney murine leukemia virus MMLV based reverse transcriptases co occur and compete with each other J Biol Chem; 294(48), 18220-18231.. PubMedID: 31640989, DOI: 10.1074/jbc.RA119.010676
- Potapov, V., Fu, X., Dai, N., Correa, I.R., Jr., Tanner, N.A., Ong, J.L (2018) Base modificatons affecting RNA polymerase and reverse transcriptase fidelity Nucleic Acids Res; 46(11): 5753-5763, PubMedID: 29750267
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