miRNA & siRNA

New England Biolabs provides many products useful for working with miRNA and siRNA.

RNA Ligases catalyze the ligation of nucleic acids through the formation of a 3'→5' phosphodiester bond. Substrates of these ligases include single-and double-stranded RNA and DNA, as well as dinucleoside pyrophosphates.

RNA ligases are routinely used for cloning and characterization of small RNAs. NEB supplies kits (NEB #E7330, NEB #E6120, NEB #E6160) containing adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing.

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Protocols for miRNA & siRNA
Reagents for RNA Sample Preparation
NEB offers a wide variety of reagents to facilitate front-end processing of RNA for downstream applications including next-generation sequencing, library construction and microarrays.
RNA Polymerase Selection Chart
NEB offers RNA polymerases that can be used for in vitro synthesis of RNA for a wide variety of downstream applications. Additional polymerases are offered for the generation of untemplated homoribopolymeric tails for reverse transcription or labeling.
RNA Ligase Selection Chart
NEB offers a variety of ligases for RNA research with unique specificities.
cDNA Synthesis Selection Chart
NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.