CutSmart_LP_Ban

CutSmart®

Return to Restriction Endonucleases

Simplify Reaction Setup and Double Digestion with CutSmart Buffer

Over 210 restriction enzymes are 100% active in a single buffer, CutSmart Buffer, making it significantly easier to set up your double digest reactions. Since CutSmart Buffer includes BSA, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in CutSmart Buffer, eliminating the need for subsequent purification.

How is NEB's new buffer system going to help me?

Although the old buffer system worked well, NEB is continuously looking for ways to enhance the convenience and performance of its products for its customers. By adding BSA to the buffer, we were able to offer > 210 enzymes that cut in a single buffer. This improves ease-of-use, especially when performing double digests. It also eliminates the need to add BSA separately.

View components of old and new buffers side by side.



Activity of DNA Modifying Enzymes in CutSmart Buffer

Enzyme
Activity in CutSmart
Required Supplements
Phosphatases:
Alkaline Phosphatase (CIP)
+ + +
 
Antarctic Phosphatase
+ + +
Requires Zn2+
Shrimp Alkaline Phosphatase (rSAP)
+ + +
 
Ligases:
T4 DNA Ligase
+ + +
Requires ATP
E. coli DNA Ligase
+ + +
Requires NAD
T3 DNA Ligase
+ + +
Requires ATP + PEG
T7 DNA Ligase
+ + +
Requires ATP + PEG
Polymerases:
T4 DNA Polymerase
+ + +
 
DNA Polymerase I, Large (Klenow) Fragment
+ + +
 
DNA Polymerase I
+ + +
 
DNA Polymerase Klenow Exo
+ + +
 
Bst DNA Polymerase
+ + +
 
phi29 DNA Polymerase
+ + +
 
T7 DNA Polymerase (unmodifed)
+ + +
 
Transferases/Kinases:
T4 Polynucleotide Kinase
+ + +
Requires ATP + DTT
T4 PNK (3´ phosphatase minus)
+ + +
Requires ATP + DTT
CpG Methyltransferase (M. SssI)
+ + +
 
GpC Methyltransferase (M. CviPI)
+
Requires DTT
T4 Phage β-glucosyltransferase (T4-BGT)
+ + +
 
Nucleases, other:
DNase I (RNase free)
+ + +
Requires Ca2+
Endonuclease III (Nth), recombinant
+ + +
 
Endonuclease VIII
+ + +
 
Exonuclease III
+ + +
 
Lambda Exonuclease
+ +
 
McrBC
+ + +
 
Micrococcal Nuclease
+ +
Requires Ca2+
RecJf
+ + +
 
T5 Exonuclease
+ + +
 
T7 Exonuclease
+ + +
 
USER® Enzyme, recombinant
+ + +
 
+ + + full functional activity + + 50–100% functional activity + 0–50% functional activity