Return to Restriction Endonucleases Products

Simplify Reaction Setup and Double Digestion with rCutSmart Buffer™

Over 210 restriction enzymes are 100% active in a single buffer, rCutSmart Buffer, making it significantly easier to set up your double digest reactions.

Since rCutSmart Buffer includes Recombinant Albumin, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in rCutSmart Buffer, eliminating the need for subsequent purification. 

View components of old and new buffers side by side.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at

Helpful Resources:


  • NEBTV_MyNEBSkill_1920

    Introducing the myNEB® Skill

    Learn how the myNEB Skill for Alexa can help life science researchers at the bench with restriction enzyme information, scientific calculations and more.