CutSmart®

Simplify Reaction Setup and Double Digestion with CutSmart Buffer

Over 215 restriction enzymes are 100% active in a single buffer, CutSmart Buffer, making it significantly easier to set up your double digest reactions.

Since CutSmart Buffer includes BSA, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in CutSmart Buffer, eliminating the need for subsequent purification. 

View components of old and new buffers side by side.

Helpful Resources:

• NEBuffer Activity/Performance Chart with Restriction Enzymes 
• Activity of DNA Modifying Enzymes in CutSmart® Buffer
• View the NEBuffer Comparison Chart to compare the components and the concentration of each component in our restriction enzyme buffers
• Cut It Out - The Restriction Enzyme Challenge from NEB
• REBASE®