Simplify Reaction Setup and Double Digestion with rCutSmart Buffer™
Over 215 restriction enzymes are 100% active in a single buffer, rCutSmart Buffer, making it significantly easier to set up your double digest reactions.
Since rCutSmart Buffer includes Recombinant Albumin, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in rCutSmart Buffer, eliminating the need for subsequent purification.
We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, NEB will be switching our current BSA-containing reaction buffers (NEBuffer™ 1.1, 2.1, 3.1 and CutSmart® Buffer) to Recombinant Albumin (rAlbumin)-containing buffers (NEBuffer r1.1, r2.1, r3.1 and rCutSmart™ Buffer). We anticipate that this switch may take as long as 6 months to complete. We feel that moving away from animal-containing products is a step in the right direction and are able to offer this enhancement at the same price. Find more details at www.neb.com/BSA-free.
During this transition period, you may receive product with BSA or rAlbumin-containing buffers. NEB has rigorously tested both and has not seen any difference in enzyme performance when using either buffer. Either buffer can be used with your enzyme. All website content will be switched in April to reflect the changes, although you may not receive the new buffer with your product immediately.
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Activity of DNA Modifying Enzymes in rCutSmart® Buffer
- View the NEBuffer Comparison Chart to compare the components and the concentration of each component in our restriction enzyme buffers
Learn how the myNEB Skill for Alexa can help life science researchers at the bench with restriction enzyme information, scientific calculations and more.