Simplify Reaction Setup and Double Digestion with rCutSmart Buffer™
Over 210 restriction enzymes are 100% active in a single buffer, rCutSmart Buffer, making it significantly easier to set up your double digest reactions.
Since rCutSmart Buffer includes Recombinant Albumin, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in rCutSmart Buffer, eliminating the need for subsequent purification.
We are excited to announce that we are almost finished with switching all reaction buffers to be BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes.for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at www.neb.com/BSA-free.
During this transition period, you may receive product with BSA or rAlbumin-containing buffers. NEB has rigorously tested both and has not seen any difference in enzyme performance when using either buffer. Either buffer can be used with your enzyme. All website content will be switched in April to reflect the changes, although you may not receive the new buffer with your product immediately.
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Activity of DNA Modifying Enzymes in rCutSmart® Buffer
- View the NEBuffer Comparison Chart to compare the components and the concentration of each component in our restriction enzyme buffers
Learn how the myNEB Skill for Alexa can help life science researchers at the bench with restriction enzyme information, scientific calculations and more.