Restriction Endonucleases
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  • RE-Mix® Restriction Enzyme Master Mixes

    Restriction enzyme digests are now even easier! The same high quality restriction enzymes that you have come to trust from New England Biolabs are now available in master mix format, including enzyme buffer and loading dye; simply add your DNA and digest for 15 minutes.

    Just add your DNA – it's that simple

    pXba DNA was digested with EcoRV-HF™ RE-Mix according to the recommended protocol. Lane L is the TriDye™ 2-LogDNA Ladder (NEB #N3270). The same results are obtained whether incubated for 5–15 minutes, 1 hour or overnight.

    Re-Mix® is a registered trademark of New England Biolabs, Inc.
    EcoRV-HF™ and TriDye™ are trademarks of New England Biolabs, Inc.

    1. 6 Reasons to Try RE-Mix Restriction Enzyme Master Mixes

    2. Setting-up Restriction Enzyme Digests with RE-Mix Master Mixes

      RE-Mix® Restriction Enzyme Master Mixes offer simplified reaction setup. Learn more about digesting DNA with RE-Mix.

    3. RE-Mix Double Digest Protocol

      Double digests are now easier than ever! Learn how to set up your next double digest with RE-Mix®.

    4. Reduce Star Activity with High-Fidelity Restriction Enzymes

      NEB has engineered HF® enzymes to eliminate star activity. Learn how, and what this means for your digests.

    5. Discovering New Restriction Enzymes

      Watch as Rick Morgan, Research Scientist in the Restriction Enzyme Division, describes his passion for discovering and characterizing restriction enzymes from nature.

    6. The Interaction of Restriction Enzymes and DNA

      Watch as Geoff Wilson, Restriction Enzyme Division Head, describes the interaction of restriction enzymes and substrate DNA using computer models generated from x-ray crystallography data.

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