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  • EcoRV-HF® RE-Mix®


    EcoRV-HF® RE-Mix® is a ready to use 10X master mix combining the restriction enzyme, EcoRV-HF (NEB #R3195 ), NEBuffer, BSA and loading dye in a single tube. Simply add DNA and water and incubate for 15 minutes to perform the restriction digest.

    Product Source

    An E. coli strain that carries the cloned and modified EcoRV gene from the plasmid J62 pLG74 (L.I. Glatman)

    Reaction Definition

    1X EcoRV-HF RE-Mix and DNA in a total reaction volume of 20 μl. Incubate at 37°C for 15 minutes.

    One reaction is defined as the amount of RE-Mix that will digest 1 μg of DNA in 15 minutes at 37°C in a total reaction volume of 20 μl.

    Properties and Usage

    Storage Temperature


    Heat Inactivation

    65°C for 20 min

    Methylation Sensitivity

    dam methylation: Not Sensitive
    dcm methylation: Not Sensitive
    CpG Methylation: Impaired by Some Combinations of Overlapping


    1. What are the advantages of using a RE-Mix Restriction Enzyme Master Mix?
    2. For some restriction enzymes star activity can be a concern. Is this also applicable to the RE-Mix?
    3. The bands on my gel are not as sharp as I would like. Is there a way to improve the sharpness?
    4. Is it possible to use RE-Mix in double digests?
    5. Can RE-Mix be used in overnight digestions?
    6. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?


    1. Standard Digest Using RE-Mix®
    2. Double Digest Protocol using Two RE-Mix® Enzymes
    3. Double Digest Protocol using One RE-Mix and One Standard Restriction Enzyme

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    Usage Guidelines & Tips

    Troubleshooting Guides

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    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.