BsmBI

Companion Products

• Nuclease-free Water
• NEB^® Golden Gate Assembly Kit (BsaI-HF^®v2)
• Esp3I
• BbsI-HF^®
• BsaI-HF®v2

Materials Sold Separately

• Gel Loading Dye, Purple (6X)
• NEBuffer 3.1

1. BsmBI is an isoschizomer of Esp3I.
2. May exhibit star activity in NEBuffer 2.1.
3. Blocked by CpG methylation.

1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes

• Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
• Activity of Restriction Enzymes in PCR Buffers
• Cleavage Close to the End of DNA Fragments
• Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
• Double Digests
• Effects of CpG Methylation on Restriction Enzyme Cleavage
• Expanded “assembly standards” for MoClo, GoldenBraid2.0 and other modular Golden Gate Assembly methods
• Heat Inactivation
• Restriction Endonucleases - Survival in a Reaction
• Restriction Enzyme Diluent Buffer Compatibility
• Restriction Enzyme Tips
• Single Letter Codes
• Star Activity
• Technical Tips For Optimizing Golden Gate Assembly Reactions

• Alphabetized List of Recognition Specificities
• Compatible Cohesive Ends and Generation of New Restriction Sites
• Cross Index of Recognition Sequences
• Dam-Dcm and CpG Methylation
• Enzymes with Nonpalindromic Sequences
• Frequencies of Restriction Sites
• Isoschizomers
• NEB Diluent and Buffer Table
• Time-Saver™ Qualified Enzymes
• Type IIS Restriction Enzymes

• DNA Sequences and Maps Tool

• Double Digest Finder
• Enzyme Finder
• NEBcutter V2.0
• NEBioCalculator
• REBASE

1. Why is there a BsmBI site in LITMUS 38 but not LITMUS 39?
2. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
3. Why is my Restriction Enzyme not cutting DNA?
4. Why do I see a DNA smear on an agarose gel after a restriction digest?
5. Why do I see additional DNA bands on my gel after a restriction digest?
6. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
7. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
8. Which restriction enzymes are used in GoldenBraid Assembly?
9. Which restriction enzymes are used in Golden Gate Assembly?
10. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?

Product Citation Tool

Additional Citations

• Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske M (2014) A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants PLoS One; 9(2), e88218. PubMedID: 24551083, DOI: 10.1371/journal.pone.0088218

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• R0580S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• R0580S_L_v1_0121403
• R0580S_L_v1_0121301
• R0580S_L_v1_0121306
• R0580S_L_v1_0121401
• R0580S_L_v1_0121407
• R0580S_L_v1_0121410
• R0580S_L_v1_0121501
• R0580S_L_v1_0121503
• R0580S_L_v1_0121507
• R0580S_L_v1_0121512
• R0580S_L_v1_0121607
• R0580S_L_v1_0121701
• R0580S_L_v1_0121706
• R0580S_L_v1_0121711
• R0580S_v1_10011886
• R0580L_v1_10011884
• R0580S_v1_10019270
• R0580S_v1_10022317
• R0580L_v1_10022315
• R0580S_v1_10027625
• R0580S_v1_10033504
• R0580S_v1_10039733
• R0580L_v1_10043213
• R0580S_v1_10043215
• R0580S_v1_10050264
• R0580L_v1_10052685
• R0580S_v1_10054267
• R0580S_v1_10056193
• R0580L_v1_10056223
• R0580L_v1_10059947
• R0580S_v1_10059950
• R0580L_v1_10063200
• R0580S_v1_10063193

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• BsmBI
• Gel Loading Dye, Purple (6X)
• NEBuffer™ 3.1

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.