PT_Product_Hero

Proteome Analysis


Proteomics using digestion of proteins followed by analysis by mass spectrometry has found wide acceptance as a way to investigate cellular expression and control. The most common protease used for digestion of proteins to produce peptides in simple or complex mixtures is Trypsin (NEB #P8101). Trypsin is treated with TPCK to destroy chymotryptic activity and acetylated on Lysine residues to prevent autolysis. Trypsin digestion produces peptides that have two positive charges in acidic solutions: the N-terminal amino group and at the C-terminal of Lysine or Arginine on the side chain. These doubly charged peptides are fragmented by Collision-Induced Disassociation (CID) and produce fingerprint spectra in a tandem mass spectrometer. A program such as Sequest or Mascot is then used to compare these spectra to the in silico predicted spectra to find the best matches if any.

Other proteases such as the Endoproteinases GluC (NEB #P8100), AspN (NEB #P8104), LysC and ArgC are also used to generate peptides that can be used in the same way. These other specificities can be used to generate larger peptides with more charges per peptide or to isolate a specific peptide when looking for a specific modification.

Trypsin-digested BSA MS Standard (CAM-modified) (NEB #P8108) is a complex mixture of peptides produced by Trypsin digestion of Bovine Serum Albumin (BSA) that was reduced and alkylated with Iodoacetamide (CAM-modified). This peptide mixture can be used to test a Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF), test a chromatographic separation or Electrospray Ionization (ESI) mass spectrometer (TOF, Q-TOF or Ion Trap).

Protocols for Proteome Analysis

    Other Tools & Resources

    Brochures

  • Glycobiology Brochure

    The Glycobiology brochure provides information on the suite of endo- and exoglycosidases, and deglycosylation enzymes offered by NEB.

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.