Protien Expression Product Hero

Affinity Purification

Many strategies can be applied to purify proteins; however, a common type of purification is affinity chromatography. This method is often preferred because it can rapidly yield pure recombinant protein in a single chromatography step. In its most typical application, a protein “tag” that has affinity for a specific immobilized substrate is genetically encoded and expressed as a fusion to the target protein. The tagged protein can then be recovered from a complex mixture (such as a cell lysate) by contacting the mixture with a chromatography resin that displays a substrate to which the tag selectively binds. Common tags include polyhistidine (His-tag), maltose binding protein (MBP-tag) and chitin binding domain (CBD-tag). 

NEB offers a variety of purification resins and beads, including nickel spin columns (NEB #S1427), nickel magnetic beads (NEB #S1423) and nickel resin (NEB #S1428) to enable rapid purification of His-tagged proteins. Amylose resins for purification of MBP-tagged proteins are available in a variety formats (standard (NEB #E0821), high flow (NEB #E0822) and magnetic (NEB #E8035), as well as Chitin resin (NEB #S6551) for rapid purification of CBD-tagged proteins

 

Choose Type:

Affinity Purification includes these subcategories:
Amylose Purification (MBP-tag)
Nickel Purification (His-tag)
Chitin Purification (CBD-tag)
FAQs for Affinity Purification
Protocols for Affinity Purification
Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please email us at busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.