PURExpress® is a reconstituted protein synthesis system based on the PUREsystem™ (Shimizu et al., 2001) where all necessary components needed for in vitro transcription and translation are purified from E. coli.
- Defined system with all His-tagged proteins for coupled transcription/translation; Ribosome is not His-tagged
- T7 RNA Polymerase drives in vitro transcription
- Minimal nuclease and protease activity for stability of synthesized protein and encoding target
- Templates can be either plasmid DNA, linear DNA or mRNA
- Protein of interest can be synthesized and visualized in a few hours
- Synthesizes various target peptides and proteins
- Synthesized protein can be co-translationally radiolabeled or fluorescently labeled
- Protein can be reverse-purified or subject to direct functional analysis
- Applications include high throughput screening/directed evolution, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity and protein-protein interactions
- Due to reconstituted nature, several kits are offered where translation factors or macromolecules have been omitted to facilitate specific studies; PURExpress® ∆ (aa, tRNA) Kit (NEB# E6840), PURExpress® ∆ RF123 Kit (NEB #E6850), PURExpress® ΔRibosome Kit (NEB #E3313)
- Compatible with the PURExpress Disulfide Bond Enhancer (NEB #E6820)
The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
- Protein Expression & Purification Brochure
- PURExpress® vs. NEBExpress® Application Chart
- Protein Expression and Purification Selection Chart
- His-Tagged Translation Factors
- Initiation Factors (IF1, IF2, IF3)
- Elongation Factors (EF-Tu, EF-Ts, EF-G)
- Release Factors (RF1, RF2, RF3)
- Ribosome Recycling Factor
- 20 Aminoacyl tRNA synthetases
- Methionyl tRNA formyltransferase
- E. coli Ribosomes
- E. coli tRNAs
- Energy Regeneration System
- NTPs, Amino Acids, Salts, Buffer
In addition, recombinant T7 RNA polymerase is used to couple transcription to translation. The PURE system represents an important step towards a totally defined in vitro transcription/translation system, thus avoiding the “black box” nature of the cell extract-based systems.
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This webinar discusses the fundamental workflows and advantages of cell-free (in vitro) protein synthesis versus traditional cellular expression methods.