IMPACT System

The IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity of protein splicing elements, termed inteins, to separate the target protein from the affinity tag.

  • Distinguishes itself from all other purification systems by its ability to purify, in a single chromatographic step, a native recombinant protein without the use of a protease
  • Able to produce a target protein without vector–derived amino acids
  • Fusion to either C-terminus or N-terminus of target protein
  • Isolation of proteins with or without an N-terminal methionine residue
  • Ligation and labeling of recombinant proteins
  • T7 promoter-driven system to achieve high levels of expression and tight transcriptional control in E. coli

Choose Type:

FAQs for IMPACT System
Protocols for IMPACT System
Application Notes for IMPACT System
Schematic illustration of the IMPACT System
Figure 2: Schematic of the IMPACT System


Flow chart for Protein Expression and Purification using the IMPACT System
Figure 5: Flow chart for Protein Expression and Purification using the IMPACT System
Sample collection for analysis by SDS-PAGE is indicated.
Intein-mediated Protein Ligation (IPL)
Figure 3: Intein-mediated Protein Ligation (IPL)


Polylinkers in the vectors pTXB1 and pTYB21
Figure 4: Polylinkers in the vectors pTXB1 and pTYB21 
▼ indicates intein cleavage site.
Purification of Maltose Binding Protein (MBP) in a single affinity purification step
Figure 1: Purification of Maltose Binding Protein (MBP) in a single affinity purification step
Lane 1: uninduced cell extract. Lane 2: induced cell extract showing expressed fusion protein. Lane 3: MBP fractions eluted after inducing cleavage overnight at 4°C. Lane 4: MBP ligated to a peptide containing an N-terminal cysteine. Marker M is the Protein Ladder (NEB #P7703).
Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please email us at busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos