Protein Expression
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  • Protein Expression & Purification Technologies

    Protein expression and purification can be very challenging because there is no single approach that is appropriate for every protein or downstream application.

    Successful expression can be affected by each individual protein’s solubility, toxicity, need for post-translational modifications or its intrinsic ability to fold. Additionally, the application for which the protein is ultimately to be used may dictate how the protein needs to be expressed or purified. It is therefore important to have a flexible set of tools and methods that can be applied to each unique project.

    Each NEB expression technology offers different advantages enabling you to choose the strategy that best suits your protein expression and purification needs. Many of the expression vectors share a compatible polylinker, enabling the gene of interest to be easily shuffled between systems. Various applications along with the recommended NEB kit can be found by clicking the sidebar tab.

    Solutions for the Expression of Difficult Proteins

    NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.

    NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.

    Featured Products

    Protein Expression & Purification Technologies includes these subcategories:

    Cell-Free Expression Information
    SHuffle strains for the expression of multi-disulfide bonded and difficult-to-express proteins
    E. coli Information
    Magnetic Matrices & Racks Information
    Yeast Information

      Publications related to Protein Expression & Purification Technologies:

    1. Sun, Z. et al. (2013). High-resolution enzymatic mapping of 5-hydroxymethylcytosine in mouse embryonic stem cells Cell Rep.. PubMedID: 23352666
    2. Lamppa, J.W., Tanyos, S.A., and Griswold, K.E. (2012). Engineering Escherichia coli for soluble expression and single step purification of active human lysozyme Journal of Biotechnology. PubMedID: 23220215, DOI: S0168-S01656
    3. Berkmen, M. (2012). Oxidative folding of proteins in Escherichia coli BioPharm International.
    4. Berkmen, M. (2012). Production of disulfide-bonded proteins in Escherichia coli Protein Expression and Purification. 240-251. PubMedID: 22085722
    5. Lobstein, J., Emrich, C.A., Jeans, C., et al. (2012). SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm Microbial Cell Factories. 11, PubMedID: 22569138
    6. Tzertzinis, G., Schildkraut, E., and Schildkraut, I. (2012). Substrate cooperativity in marine luciferases PLoS One. 7, PubMedID: 22768230
    7. Samuelson, J.C., Causey, T.B., and Berkmen, M. (2012). Disulfide-bonded protein expression in E. coli Genetic Engineering & Biotechnology News. 32(3), 35.
    8. Sawada, R. et al. (2011). A phage-targeting startegy for the design of spatiotemporal drug delivery from grafted matrices Fibrogenesis Tissue Repair. 4, 7. PubMedID: 17641242
    9. Ganatra, M.B., Rainauskas, S., Hong J.M., Taylor, T.E., Denson, J.P.M., Esposito, D., Read, J.D., Schmeisser, H., Zoon, K.C., Hartley, J.L. and Taron, C.H. (2011). A set of aspartyl protease-deficient strains for improved expression of heterologous proteins in Kluyveromyces lactis FEMS Yeast Res.. 11, 168-178. PubMedID: 21166768
    10. Whitney, M.A. et al. (2011). Fluorescent peptides highlight peripheral nerves during surgery in mice Nat. Biotechnol. . 6, PubMedID: 21297616
    11. Ritter, A. (2011). Next-gen expression systems. 35, 36-39. Pharnaceutical Technology.
    12. Tait, A.R. and Straus, S.K. (2011). Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconvential use of oxidizing environments with a maltose binding protein-hexahistidine dual tag to enhance membrane protein yield Microbial Cell Factories. 10, PubMedID: 21130169
    13. Zhi, M. et al. (2011). Screening of specific binding peptide targeting blood vessel of human esophageal cancer in vivo in mice Chin. Med. J. . 124, 581-585. PubMedID: 21362285
    14. Salinas, G., Pellizza, L., Margenat, M., et al. (2011). Tuned Escherichia coli as a host for the expression of disulfide-rich proteins Biotechnology Journal. 6, 689-699. PubMedID: 21567960
    15. Correa, A. and Oppezzo, P. (2011). Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: Advantages of high-throughput screening Biotechnology Journal. 6, 715-730. PubMedID: 21567962
    16. Ganatra, M.B., Rainauskas, S., Hong, J.M., Taylor, T.E., Denson, J.P.M., Esposito, D., Read, J.D., Schmeisser, H., Zoon, K.C., Hartley, J.L. and Taron, C.H. (2011). A set of aspartyl protease-deficient strains for improved expression of heterologous proteins in Kluyveromyces lactis FEMS Yeast Res.. 11, 168-178. PubMedID: 21166768
    17. Feng, Z., et al. (2011). Disruption of PMR1 in Kluyveromyces lactis improves secretion of calf prochymosin J. Sci. Food Agric.. 15, 100-103.
    18. Narayan, V., Pion, E., Landré, V., Müller, P. and Ball, K.L. (2011). Docking-dependent ubiquitination of the interferon regulatory factor-1 tumor suppressor protein by the ubiquitin ligase CHIP J. Biol. Chem. . 286, 607-619. PubMedID: 20947504
    19. Rosner, K., Kasprzak, M.F., Horenstein, A.C., Thurston, H.L., Abrams, J., Kerwin, L.Y., Mehregan, D.A. and Mehregan, D.R. (2011). Engineering a waste management enzyme to overcome cancer resistance to apoptosis: adding DNase1 to the anti-cancer toolbox Cancer Gene Ther. . 18, 346-357. PubMedID: 21233855
    20. Zhou, X., et al. (2011). Expression of heparin sulfate sulfotransferases in Kluyveromyces lactisand preparation of PAPS Glycobiology Advance Access.
    21. Rocha S.N., et al. (2011). Heterologous expression of a thermophilic esterase in Kluyveromyces yeasts Appl. Microbiol. Biotechnol.. 89, 375-85. PubMedID: 20862582
    22. Yamamoto, S., Izumiya, H., Mitobe, J., Morita, M., Arakawa, E., Ohnishi, M. and Watanabe, H. (2011). Identification of a chitin-induced small RNA that regulates translation of the tfoX gene, encoding a positive regulator of natural competence in Vibrio cholerae J Bacteriol. . 193, 1953-1965. PubMedID: 21317321
    23. Iizuka, R., Yamagishi-Shirasaki, M. and Funatsu, T. (2011). Kinetic study of de novo chromophore maturation of fluorescent proteins Anal. Biochem. . Apr 1., PubMedID: 21459075
    24. Midon, M., Schäfer, P., Pingoud, A., Ghosh, M., Moon, A.F., Cuneo, M.J., London, R.E. and Meiss, G. (2011). Mutational and biochemical analysis of the DNA-entry nuclease EndA from Streptococcus pneumoniae Nucleic Acids Res. . 39, 623-634. PubMedID: 20846957
    25. Feng, Z., et al. (2011). Optimization of Medium Composition for Production of Recombinant Calf Chymosin from Kluyveromyces lactis in Submerged Fermentation J. of Northeast Agricultural University. 18, 56-62.
    26. Lamichhane, T.N., Abeydeera, N.D., Duc, A.C., Cunningham, P.R. and Chow, C.S (2011). Selection of peptides targeting helix 31 of bacterial 16S ribosomal RNA by screening M13 phage-display libraries Molecules . 16, 1211-1239. PubMedID: 21278676
    27. Panayiotou, C., Solaroli, N., Xu, Y., Johansson, M. and Karlsson, A. (2011). The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes Biochem J.  . 2433, 527-534. PubMedID: 21080915
    28. De Masi, F., Grove, C.A., Vedenko, A., Alibés, A., Gisselbrecht, S.S., Serrano, L., Bulyk, M.L. and Walhout, A.J. (2011). Using a structural and logics systems approach to infer bHLH-DNA binding specificity determinants Nucleic Acids Res. . 18-Feb, PubMedID: 21335608
    29. Handa, Y., Inaho, N. and Nameki, N. (2011). YaeJ is a novel ribosome-associated protein inEscherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes Nucleic Acids Res.  . 39, 1739-1748. PubMedID: 21051357
    30. Ohashi, H., Kanamori, T., Shimizu, Y. and Ueda, T. (2010). A highly controllable reconstituted cell-free system--a breakthrough in protein synthesis research Curr. Pharm. Biotechnol.. 11, 267-271. PubMedID: 20210745
    31. Wu, D. et al. (2010). Anti-tumor effects of a novel chimeric peptide on S180 and H22 xenografts bearing nude mice Peptides . 31, 850-864. PubMedID: 20132854
    32. Welch, B.D. et al. (2010). Design of a potent D-peptide HIV-1 entry inhibitor with a strong barrier to resistance J. Virol.. 84, 11235-11244. PubMedID: 20719956
    33. Wu, C.C. et al. (2010). Identification of a new peptide for fibrosarcoma tumor targeting and imaging in vivo J. Biomed. Biotechnol. . PubMedID: 21151669
    34. Seow, Y. et al. (2010). Identification of a novel muscle targeting peptide in mdx mice Peptides. 31, PubMedID: 20621144
    35. Zhao, W. et al. (2010). Isolation and initial application of a novel peptide that specifically recognizes the neural stem cells derived from rhesus monkey embryonic stem cells J. Biomol. Screen.. 15, 687-694. PubMedID: 20508256
    36. Zhao, S. et al. (2010). Novel peptide ligands that bind specifically to mouse embryonic stem cells Peptides. 31, 2027-2034. PubMedID: 20713104
    37. Sun, X. et al. (2010). Phage display-derived peptides for osteosarcoma imaging Clin. Cancer Res.. 16, 4268-4277. PubMedID: 20570932
    38. Yoon, S.H., Kim, S.K., and Kim J.F. (2010). Secretory production of recombinant proteins in Escherichia coli Recent Patents on Biotechnology. 4, 23-29. PubMedID: 20201800
    39. Karukurichi, K. et al. (2010). Analysis of p300/CBP histone acetyltransferase regulation using circular permutation and semisynthesis J. Am. Chem. Soc. . 132, 1222- 1223. PubMedID: 20063892
    40. Chen, J., et al. (2010). Chemically ubiquitylated PCNA as a probe for eukaryotic translesion DNA synthesis Nat. Chem. Biol. . 6, 270-272. PubMedID: 20208521
    41. Collins, E. D., et. al. (2010). Cloning the human vitamin D receptor into the pTwin-1 expression vector J Steroid Biochem Mol Biol. PubMedID: 20171280
    42. Feng, Z., et al. (2010). Codon optimization of the calf prochymosin gene and its expression in Kluyveromyces lactis World J. Microbiol. Biotechnol.. 26, 895-901.
    43. Lu, X., et al. (2010). Designed semisynthetic protein inhibitors of ub/ubl e1 activating enzymes J. Am. Chem. Soc. . 132, 1748-1749. PubMedID: 20099854
    44. Kuo, D., et al. (2010). Evolutionary divergence in the fungal response to fluconazole revealed by soft clustering Gen. Bio. . 11, R77. PubMedID: 20653936
    45. Yu, H. H., et. al. (2010). Expressed protein ligation for the preparation of fusion proteins with cell penetrating peptides for endotoxin removal and intracellular delivery Biochim Biophys Acta . PubMedID: 20170629
    46. Manconi, B., et. al. (2010). Expression, purification, phosphorylation and characterization of recombinant human statherin Protein Expr Purif.. 69, 219-225.
    47. Yuan W, et al. (2010). Gene synthesis of the bovine prochymosin gene and high-level expression in Kluyvermyces lactis Sheng Wu Gong Cheng Xue Bao. 26, 1281-1286. PubMedID: 21141120
    48. Rocha, S.N., et al. (2010). Heterologous expression of glucose oxidase in the yeastKluyveromyces marxianus Microb. Cell Fact.. 21, PubMedID: 20092622
    49. Asahara, H. and Chong, S. (2010). In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme Nucleic Acids Res. . 38, e141. PubMedID: 20457746
    50. Lu, M., et. al. (2010). Purification of untagged HIV-1 reverse transcriptase by affinity chromatography Protein Expr. Purif.. 71, 231-239. PubMedID: 20060474
    51. Ueda, T., Kanamori, T., Ohashi, H. (2010). Ribosome display with the PURE technology Methods Mol Biol. . 607, 219-25. PubMedID: 20204860
    52. Theerthagiri, G., Eisenhardt, N., Schwarz, H. and Antonin, W. (2010). The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex  J. Cell Biol.  . 189, 1129-1142. PubMedID: 20566687
    53. Noto, T., Kurth, H., Kataoka, K., Aronica, L., DeSouza, L., Siu, K., Pearlman, R., Gorovsky, M. and Mochizuki, K. (2010). The tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus Cell . 140, 692-703. PubMedID: 20211138
    54. Shen, B.W., Heiter, D.F., Chan, S.H., Wang, H., Xu, S.Y., Morgan, R.D., Wilson, G.G. and Stoddard, B.L. (2010). Unusual target site disruption by the rare-cutting HNH restriction endonuclease PacI Structure . 18, 734-743. PubMedID: 20541511
    55. Nothelfer, E.M. et al. (2009). Identification and characterization of a peptide with affinity to head and neck cancer J. Nucl. Med.. 50, 426-434. PubMedID: 19223422
    56. Wan, X.M. et al. (2009). Identification of nose-to-brain homing peptide through phage display Peptides. 30, 19007831. PubMedID: 343-350
    57. de Marco, A. (2009). Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli Microbial Cell Factories. 8, PubMedID: 19442264
    58. Mueller, J. et al. (2009). Targeting of tumor blood vessels: a phage-displayed tumor-homing peptide specifically binds to matrix metalloproteinase-2-processed collagen IV and blocks angiogenesis in vivo Mol. Cancer Res.. 7, 1078-1085. PubMedID: 19584266
    59. Feng, Y. and Cronan, J. E. (2009). A new member of the Eschericia coli fad regulon: transcriptional regulation of fadM (ybaW) J. Bacteriol. . 191, 6320-6328. PubMedID: 19684132
    60. Feng, Z., et al (2009). Effect of temperature-shift strategy on recombinat chymosin of Kluyveromyces Lactis Heilongjiang Animal Science and Veterinary Medicine. 2009-2013.
    61. Tanner, D., Cariello, D., Woolstenhulme, C., Broadbent, M. and Buskirk, A. (2009). Genetic identification of nascent peptides that induce ribosome stalling J. Biol. Chem. . 284, 34809-34818. PubMedID: 19840930
    62. Solaroli, N., Panayiotou, C., Johansson, M., and Karlsson, A. (2009). Identification of two active functional domains of human adenylate kinase 5 FEBS Lett. . 583, 2872-2876. PubMedID: 19647735
    63. Talabot-Ayer, D., Lamacchia, C., Gabay, C., and Palmer, G. (2009). Interleukin-33 is biologically active independently of Caspase-1 cleavage J. Biol. Chem. . 284, 19420-19426. PubMedID: 19465481
    64. Estevez-Torres, A., Crozatier, C., Diguet, A., Hara, T., Saito, H., Yoshikawa, K., and Baigl, D. (2009). Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder Proc. Natl. Acad. Sci.  . 106, 12219-12223. PubMedID: 19617550
    65. Chattopadhaya, S., et al. (2009). Site-specific covalent labeling of proteins inside live cells using small molecule probes Bioorg. Med. Chem.. 17, 981-9. PubMedID: 18261914
    66. Che, N., et. al. (2009). Soluble expression and one-step purification of a neurotoxin Huwentoxin-I in Escherichia coli Protein Expr Purif.. 65, 154-159. PubMedID: 19217942
    67. Madinger, C.L., Sharma, S.S., Anton, B.P., Fields, L.G., Cushing, M.L., Canovas, J., Taron, C.H. and Benner, J.S. (2009). The effect of carbon source on the secretome of Kluyveromyces lactis Proteomics. 9, 4744-4754. PubMedID: 19743416
    68. Chattopadhaya, S., et al. (2009). Use of intein-mediated protein ligation strategies for the fabrication of functional protein arrays Methods Enzymol.. 462, 195-223. PubMedID: 19632476
    69. Hondal, R. J. (2009). Using chemical approaches to study selenoproteins-Focus on thioredoxin reductases Biochim. Biophys. Acta.. 1790, 1501-1512. PubMedID: 19406205
    70. Zheng, Y., Posfai, J., Morgan, R. D., Vincze, T., and Roberts, R.J. (2009). Using shotgun sequence data to find active restriction enzyme genes Nuc. Acid. Res. . 37, el. PubMedID: 18988632
    71. Ottesen, J. et al. (2008). An amalgamation of solid phase peptide synthesis and ribosomal peptide synthesis Biopolymers. 90, 406-414. PubMedID: 17636509
    72. Zhao, W. et al. (2008). An efficient on-column expressed protein ligation strategy: application to segmental triple labeling of human apolipoprotein E3 Protein Sci. . 17, 736-747. PubMedID: 18305193
    73. Kazuta, Y., Adachi, J., Matsuura, T., Ono, N., Mori, H. and Yomo T. (2008). Comprehensive Analysis of the Effects of Eschericia coli ORF's on Protein Translation Reaction Molecular & Cellular Proteomics . 7, 1530-1540. PubMedID: 18453339
    74. Babu, S.K., et. al. (2008). Construction of intein mediated hGMCSF expression vector and its prufication in Pichia pastoris Protein Exp. Purif.. 57, 201-205. PubMedID: 18309571
    75. Chen, Y. Q., et. al. (2008). Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners Protein Exp. Purif.. 57, 303-311. PubMedID: 17977015
    76. Platko, J.D., et al. (2008). Heterologous expression of Mytilus californianus foot protein three (Mcfp-3) in Kluyveromyces lactis Platko, J.D., et al.. 57, 57-62. PubMedID: 17923416
    77. Skrisovska, L. et al. (2008). Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L. J. Mol. Biol.. 375, 151-164. PubMedID: 17936301
    78. Vazquez-Laslop, N., Thum, C., and Mankin, A.S. (2008). Molecular Mechanism of Drug-Dependent Ribosome Stalling  Mol. Cell. . 30, 190-202. PubMedID: 18439898
    79. Swaim, C.L., et al. (2008). Physical and computational analysis of the yeast Kluyveromyces lactissecreted proteome Proteomics. 8, 2714-2723. PubMedID: 18601269
    80. Zhao, Z., et. al. (2008). Purification of green fluorescent protein using a two-intein system Appl. Microbiol. Biotechnol.. 77, 1175-1180. PubMedID: 17973109
    81. Subtelny. A. O.,Hartman, M. C. T., and Szostak, J. W. (2008). Ribosomal Synthesis of N-Methyl Peptides  J. Am. Chem. Soc. . 130, 6131-6136. PubMedID: 18402453
    82. Sako, Y., Goto, Y., Murakami, H., and Suga, H. (2008). Ribosomal Synthesis of Peptidase-Resistant Peptides Closed by a Nonreducible Inter-Side-Chain Bond ACS Chem. Biol.  . 3, 241-249. PubMedID: 18338852
    83. Takakuwa, N., Oshnishi, M., and Oda, Y. (2008). Significance of the KLAC1 gene in glucosylceramide production by Kluyveromyces lactis FEMS Yeast Res. 9, 839-846. PubMedID: 18631186
    84. Uemura, S., Iizuka, R., Ueno, T., Shimizu, Y., Taguchi, H., Ueda, T., Puglisi. J., and Funatsu, T. (2008). Single molecule imaging of full protein synthesis by immobilized ribosomes Nuc. Acid. Res.  . 36, e90. PubMedID: 18511463
    85. Sugiki, T. Shimada, I. and Takahashi H. (2008). Stable isotope labeling of protein byKluyveromyces lactis for NMR study J. Biomol NMR.. 42, 159-62. PubMedID: 18827973
    86. Foster, J.M., Raverdy, S., Ganatra, M.B., Colussi, P.A., Taron, C.H. and Carlow, C.K.S. (2008). The Wolbachia endosymbiont of Brugia malayi has an active phosphoglycerate mutase: a candidate target for anti-filarial therapies Parasitol. Res. . PubMedID: 19043737
    87. Zitzman, S. et al. (2007). Identification and evaluation of a new tumor cell-binding peptide J. Nucl. Med.. 48, 965-972. PubMedID: 17504878
    88. Schmidt, A. et al. (2007). Selecting targeting of adenoviral vectors to neural precursor cells in the hippocampus of adult mice: new prospects for in situ gene therapy Stem Cells. 25, 2910-2918. PubMedID: 17641242
    89. Stasser, J. P., et. al. (2007). A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase Biochem. . 46, 11845-11856. PubMedID: 17902702
    90. Hassiepen, U., et. al. (2007). A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine110-glycine as substrate Anal Biochem.. 371, 201-207. PubMedID: 17869210
    91. Sharma, C.M., Darfeuille, F., Plantinga, T.H., and Vogel, J. (2007). A small RNA regulates multiple ABC transporter mRNA's by targeting C/A-rich elements inside and upstream of ribosome-binding sites Gene Dev.. 21, 2804-2817. PubMedID: 17974919
    92. Read, J.D., Colussi, P.A., Ganatra, M.B. and Taron, C.H. (2007). Acetamide selection of Kluyveromyces lactis cells transformed with an integrative vector leads to high frequency formation of multicopy strains Appl. Environ. Microbiol.. 73, 5088-5096. PubMedID: 17586678
    93. Karow, A., et al. (2007). Authentic interdomain communication in an RNA helicase reconstituted by expressed protein ligation of two helicase domains FEBS J. . 2, 463-473. PubMedID: 17229151
    94. Zhao, H.L., et al. (2007). Circumventing the heterogeneity and instability of human serum albumin-interferon-_2b fusion protein by altering its orientation J. Biotech. . 131, 245-252. PubMedID: 17698234
    95. Sun, L., et al. (2007). Design, preparation and use of ligated phosphoproteins: A novel approach to study protein phosphatases by dot blot array, ELISA and Western blot assays Methods. 42, 220-226. PubMedID: 17532508
    96. Hauser, P. S., et al. (2007). Expressed protein ligation using an N-terminal cysteine containing fragment generated in vivo from a pelB fusion protein Protein Expr. Purif.. 54, 227-233. PubMedID: 17493830
    97. Wamalwa, B.M., et al. (2007). High-Level Heterologous Expression of Bacillus halodurans Putative Xylanase Xyn11A (BH0899) in Kluyveromyces lactis Biosci. Biotechnol. Biochem.. 71, 688-693. PubMedID: 17341817
    98. Diao, H., et. al. (2007). Intein-mediated expression is an effective approach in the study of beta-defensins Biochem Biophys Res Commun. 357, 840-846. PubMedID: 17445764
    99. Bhat, R. K. and Berger, S. (2007). New and easy strategy for cloning, expression, purification, and characterization of the 5S subunit of transcarboxylase from Propionibacterium f. shermanii Prep. Biochem. Biotechnol.. 37, 13-26. PubMedID: 17134979
    100. Murtas, G., Kuruma, Y., Bianchini, P., Diaspro, A., and Luisi, P.L. (2007). Protein synthesis in liposomes with a minimal set enzymes Biochem. Biophys. Res. Commun.  . 363, 12-17. PubMedID: 17850764
    101. Ohta, A., Murakami, H., Higashimura, E., and Suga, H. (2007). Synthesis of Polyester by Means of Genetic Code Reprogramming Chem. Biol. . 14, 1315-1322. PubMedID: 18096500
    102. Paulick, M. G., et al. (2007). Synthetic analogues of glycosylphosphatidylinositol-anchored proteins and their behavior in supported lipid bilayers J. Am. Chem. Soc. . 129, 11543-11550. PubMedID: 17715922
    103. Kochinyan, S., et al. (2007). Use of intein-mediated phosphoprotein arrays to study substrate specificity of protein phosphatases Biotechniques. 42, 63-69. PubMedID: 17269486
    104. Hackenberger, C. P., et. al. (2006). Expression of N-terminal Cys-protein fragments using an intein refolding strategy Bioorg. Med. Chem.. 14, 5043-5048.
    105. Muto, H., Nakatogawa, H., and Ito, K. (2006). Genetically Encoded but Nonpolypeptide Prolyl-tRNA Functions in the A Site for SecM-Mediated Ribosomal Stall Mol. Cell.. 22, 545-552. PubMedID: 16713584
    106. Van Ooyen, A.J.J., Dekker, P., Huang, M., Olsthoorn, M.M.A., Jacobs, D.I., Colussi P.A., and Taron, C.H. (2006). Heterologous protein production in the yeast Kluyveromyces lactis FEMS Yeast Res.. 6, 381-392. PubMedID: 16630278
    107. Villemagne, D., Jackson, R. and Douthwaite, J.A. (2006). Highly efficient ribosome display selection by use of purified components for in vitro translation J. Immunol Methods. 313, 140-148. PubMedID: 16730021
    108. Gottlieb, D., et al. (2006). Intein-mediated in vitro synthesis of lipidated Ras proteins Chem. Commun. (Camb). 21, 260-262. PubMedID: 16391727
    109. Wojtaszek, J., et al. (2006). LT CI, a novel chymotrypsin inhibitor of the potato I family from the earthworm Lumbricus terrestris. Purification, cDNA cloning, and expression Comp. Biochem. Physiol B. Biochem. Mol. Biol. . 143, 465-472.
    110. Esipov, R.S., et. al. (2006). Production and purification of recombinant human glucagons overexpressed as intein fusion protein in Escherichia coli Protein Pept. Lett.. 13, 343-347. PubMedID: 16712508
    111. Demidov, V.V., and Broude, N.E. (2006). Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro Nat. Protoc.. 1, 714-719. PubMedID: 17406301
    112. Kalia, J., et al. (2006). Reactivity of intein thioesters: appending a functional group to a protein Chembiochem.. 7, 1375-1383. PubMedID: 16897799
    113. Kaiser, C.M., Chang, H.C., Agashe, V.R., Lakshmipathy, S.K., Etchells, S.A., Hayer-Hartl, M., Hartl, F.U. and Barral, J.M. (2006). Real-time observation of trigger factor function on translating ribosomes Nature. 444, 455-460. PubMedID: 17051157
    114. Eckenroth, B., et al. (2006). Semisynthesis and characterization of mammalian thioredoxin reductase Biochem.. 45, 5158-70. PubMedID: 16618105
    115. Shimizu, Y. and Ueda, T. (2006). SmpB triggers GTP hydrolysis of elongation factor Tu on ribosome by compensating for the lack of codon-anticodon interaction during trans-translation initiation J. Biol. Chem.. 281, 15987-15996. PubMedID: 16601123
    116. Lee, N.Y., et al. (2006). Structure and dynamics of the epidermal growth factor receptor C-terminal phosphorylation domain Protein Sci.. 15, 1142-52. PubMedID: 16597832
    117. Grant, J., et al. (2006). The N terminus of GTP gamma S-activated transducin alpha-subunit interacts with the C terminus of the cGMP phosphodiesterase gammasubunit J. Biol. Chem. . 281, 6194-6202. PubMedID: 16407279
    118. Sando, S., Kanatani, K., Sato, N., Matsumoto, H. Hohsaka, T. and Aoyama, Y. (2005). A small-molecule-based approach to sense codon-templated natural-unnatural hybrid peptides. Selective silencing and reassignment of the sense codon by orthogonal reacylation stalling at the single-codon level J. Am. Chem. Soc.. 127, 7998-7999. PubMedID: 15926808
    119. Ingham, A.B., et al. (2005). A versatile system for the expression of nonmodified bacteriocins inEscherichia coli J. Appl. Microbiol. . 98, 676-683. PubMedID: 15715871
    120. Colussi, P.A., Specht, C.A. and Taron, C.H. (2005). Characterization of a nucleus-encoded chitinase from the yeast Kluyveromyces lactis Appl. Environ. Microbiol.. 71, 2862-2869. PubMedID: 15932978
    121. Ying, B.W., Taguchi, H., Kondo, M. and Ueda, T. (2005). Co-translational involvement of the chaperonin GroEL in the folding of newly translated polypeptides J. Biol. Chem.. 280, 12035-12040. PubMedID: 15664980
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    Protein Expression and Purification: Selection Chart

    Each NEB expression technology offers different advantages which enable you to choose the strategy that best suits your protein expression and purification needs.