Protein Expression
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  • Protein Expression & Purification Technologies

    Protein expression and purification can be very challenging because there is no single approach that is appropriate for every protein or downstream application.

    Successful expression can be affected by each individual protein’s solubility, toxicity, need for post-translational modifications or its intrinsic ability to fold. Additionally, the application for which the protein is ultimately to be used may dictate how the protein needs to be expressed or purified. It is therefore important to have a flexible set of tools and methods that can be applied to each unique project.

    Each NEB expression technology offers different advantages enabling you to choose the strategy that best suits your protein expression and purification needs. Many of the expression vectors share a compatible polylinker, enabling the gene of interest to be easily shuffled between systems. Various applications along with the recommended NEB kit can be found by clicking the sidebar tab.

    Solutions for the Expression of Difficult Proteins

    NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.

    NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.

    Featured Products

    Protein Expression & Purification Technologies includes these subcategories:

    Cell-Free Expression Information
    E. coli Information
    Magnetic Matrices & Racks Information
    Yeast Information

      Publications related to Protein Expression & Purification Technologies:

    1. Zitzman, S. et al. (2007) Identification and evaluation of a new tumor cell-binding peptide J. Nucl. Med. 48, 965-972. PubMedID: 17504878
    2. Schmidt, A. et al. (2007) Selecting targeting of adenoviral vectors to neural precursor cells in the hippocampus of adult mice: new prospects for in situ gene therapy Stem Cells 25, 2910-2918. PubMedID: 17641242
    3. Nothelfer, E.M. et al. (2009) Identification and characterization of a peptide with affinity to head and neck cancer J. Nucl. Med. 50, 426-434. PubMedID: 19223422
    4. Wan, X.M. et al. (2009) Identification of nose-to-brain homing peptide through phage display Peptides 30, 19007831. PubMedID: 343-350
    5. de Marco, A. (2009) Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli Microbial Cell Factories 8, PubMedID: 19442264
    6. Mueller, J. et al. (2009) Targeting of tumor blood vessels: a phage-displayed tumor-homing peptide specifically binds to matrix metalloproteinase-2-processed collagen IV and blocks angiogenesis in vivo Mol. Cancer Res. 7, 1078-1085. PubMedID: 19584266
    7. Ohashi, H., Kanamori, T., Shimizu, Y. and Ueda, T. (2010) A highly controllable reconstituted cell-free system--a breakthrough in protein synthesis research Curr. Pharm. Biotechnol. 11, 267-271. PubMedID: 20210745
    8. Wu, D. et al. (2010) Anti-tumor effects of a novel chimeric peptide on S180 and H22 xenografts bearing nude mice Peptides 31, 850-864. PubMedID: 20132854
    9. Welch, B.D. et al. (2010) Design of a potent D-peptide HIV-1 entry inhibitor with a strong barrier to resistance J. Virol. 84, 11235-11244. PubMedID: 20719956
    10. Wu, C.C. et al. (2010) Identification of a new peptide for fibrosarcoma tumor targeting and imaging in vivo J. Biomed. Biotechnol. PubMedID: 21151669
    11. Seow, Y. et al. (2010) Identification of a novel muscle targeting peptide in mdx mice Peptides 31, PubMedID: 20621144
    12. Zhao, W. et al. (2010) Isolation and initial application of a novel peptide that specifically recognizes the neural stem cells derived from rhesus monkey embryonic stem cells J. Biomol. Screen. 15, 687-694. PubMedID: 20508256
    13. Zhao, S. et al. (2010) Novel peptide ligands that bind specifically to mouse embryonic stem cells Peptides 31, 2027-2034. PubMedID: 20713104
    14. Sun, X. et al. (2010) Phage display-derived peptides for osteosarcoma imaging Clin. Cancer Res. 16, 4268-4277. PubMedID: 20570932
    15. Yoon, S.H., Kim, S.K., and Kim J.F. (2010) Secretory production of recombinant proteins in Escherichia coli Recent Patents on Biotechnology 4, 23-29. PubMedID: 20201800
    16. Sawada, R. et al. (2011) A phage-targeting startegy for the design of spatiotemporal drug delivery from grafted matrices Fibrogenesis Tissue Repair 4, 7. PubMedID: 17641242
    17. Ganatra, M.B., Rainauskas, S., Hong J.M., Taylor, T.E., Denson, J.P.M., Esposito, D., Read, J.D., Schmeisser, H., Zoon, K.C., Hartley, J.L. and Taron, C.H. (2011) A set of aspartyl protease-deficient strains for improved expression of heterologous proteins in Kluyveromyces lactis FEMS Yeast Res. 11, 168-178. PubMedID: 21166768
    18. Whitney, M.A. et al. (2011) Fluorescent peptides highlight peripheral nerves during surgery in mice Nat. Biotechnol. 6, PubMedID: 21297616
    19. Ritter, A. (2011) Next-gen expression systems 35, 36-39. Pharnaceutical Technology.
    20. Tait, A.R. and Straus, S.K. (2011) Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconvential use of oxidizing environments with a maltose binding protein-hexahistidine dual tag to enhance membrane protein yield Microbial Cell Factories 10, PubMedID: 21130169
    21. Zhi, M. et al. (2011) Screening of specific binding peptide targeting blood vessel of human esophageal cancer in vivo in mice Chin. Med. J. 124, 581-585. PubMedID: 21362285
    22. Salinas, G., Pellizza, L., Margenat, M., et al. (2011) Tuned Escherichia coli as a host for the expression of disulfide-rich proteins Biotechnology Journal 6, 689-699. PubMedID: 21567960
    23. Correa, A. and Oppezzo, P. (2011) Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: Advantages of high-throughput screening Biotechnology Journal 6, 715-730. PubMedID: 21567962
    24. Samuelson, J.C., Causey, T.B., and Berkmen, M. (2012) Disulfide-bonded protein expression in E. coli Genetic Engineering & Biotechnology News
    25. Lamppa, J.W., Tanyos, S.A., and Griswold, K.E. (2012) Engineering Escherichia coli for soluble expression and single step purification of active human lysozyme Journal of Biotechnology PubMedID: 23220215, DOI: S0168-S01656
    26. Berkmen, M. (2012) Oxidative folding of proteins in Escherichia coli BioPharm International
    27. Berkmen, M. (2012) Production of disulfide-bonded proteins in Escherichia coli Protein Expression and Purification 240-251. PubMedID: 22085722
    28. Lobstein, J., Emrich, C.A., Jeans, C., et al. (2012) SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm Microbial Cell Factories 11, PubMedID: 22569138
    29. Tzertzinis, G., Schildkraut, E., and Schildkraut, I. (2012) Substrate cooperativity in marine luciferases PLoS One 7, PubMedID: 22768230
    30. Sun, Z. et al. (2013) High-resolution enzymatic mapping of 5-hydroxymethylcytosine in mouse embryonic stem cells Cell Rep. PubMedID: 23352666
    31. Zahid, M. et al. Identification of a cardiac specific protein transduction domain by in vivo biopanning using a M13 phage peptide display library in mice PLoS One 5, PubMedID: 20808875
    32. Vernet, E., Kotzsch, A., Voldborg, B., et al. Screening of genetic parameters for soluble protein expression in Escherichia coli Protein Expression and Purification 77, 104-111. PubMedID: 21130169
    33. Cotton, G.J. and Muir, T.W. (2000) Generation of a dual-labeled fluorescence biosensor for Crk-II phosphorylation using solid-phase expressed protein ligation Chem. Biol 4, 253-261. PubMedID: 10780925
    34. Shimizu, Y., Inoue, A., Tomari, Y., Suzuki, T., Yokogawa, T., Nishikawa, K. and Ueda, T. (2001) Cell-free translation reconstituted with purified components Nat. Biotech. 19, 751-755. PubMedID: 11479568
    35. Wu, J.W., et al. (2001) Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and insights on Smad function in TGF-beta signaling Mol Cell. 8, 1277-1289. PubMedID: 11779503
    36. Hanzawa, H., et al. (2001) In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore Proc. Natl. Acad. Sci. USA 98, 3612-3617. PubMedID: 11248126
    37. Dorsch, S., et al. (2001) The VP1-unique region of parvovirus B19: amino acid variability and antigenic stability J. Gen. Virol. 82 (pt 1), 191-199. PubMedID: 11125172
    38. Wojciechowski CL, Cardia JP, and Kantrowitz ER. (2002) Alkaline phosphatase from the hyperthermophilic bacterium T. maritima requires cobalt for activity Protein Sci 4, 903-911. PubMedID: 11910033
    39. Wojciak, J.M., et al. (2002) Arm-site binding by lambda -integrase: solution structure and functional characterization of its amino-terminal domain Proc. Natl. Acad. Sci. USA 99, 3434-3439. PubMedID: 11904406
    40. Camarero, J.A., et al. (2002) Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR Proc. Natl. Acad. Sci. USA 99, 8536-8541. PubMedID: 12084914
    41. Moolenaar GF, et al. (2002) Cho, a second endonuclease involved in Escherichia coli nucleotide excision repair Proc. Natl. Acad. Sci. US 99, 1467-1472. PubMedID: 11818552
    42. Zhong, Q., et al. (2002) Endosomal localization and function of sorting Proc. Natl. Acad. Sci. USA 99, 6767-6772.
    43. Humphries, H. E., Christodoulides, M. and Heckels, J. E. (2002) Expression of the class 1 outer-membrane protein of Neisseria meningitidis in Escherichia coli and purification using a self-cleavable affinity tag Protein Expr. Purif.  26, 243-8.
    44. Singleton, S. F., et al. (2002) Intein-mediated affinity-fusion purification of the Escherichia coliRecA protein Protein Expr. Purif.  26, 476-488.
    45. Nakano, S., et al. (2002) Multiple pathways of Spx (YjbD) proteolysis in Bacillus subtilis J. Bacteriol 184, 3664-3670. PubMedID: 12057962
    46. Hong, S.H., and Maret, W. (2003) A fluorescence resonance energy transfer sensor for the beta-domain of metallothionein Proc. Natl. Acad. Sci. USA 100, 2255-2260. PubMedID: 12618543
    47. Schaffer, L., et al. (2003) Assembly of high-affinity insulin receptor agonists and antagonists from peptide building blocks Proc. Natl. Acad. Sci. USA 100, 4435-9. PubMedID: 12684539
    48. Salazar, J.C., et al. (2003) Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates Proc. Natl. Acad. Sci. USA 100, 13863-13868. PubMedID: 14615592
    49. Walters, K.J., et al. (2003) DNA -repair protein hHR23a alters its protein structure upon binding proteasomal subunit S5a Proc. Natl. Acad. Sci. USA 100, 12694-12699.
    50. Yee, C.S., et al. (2003) Generation of the R2 subunit of ribonucleotide reductase by intein chemistry: insertion of 3-nitrotyrosine at residue 356 as a probe of the radical initiation process Biochemistry 42, 14541-14552. PubMedID: 14661967
    51. Wuebbens, M. M. and Rajagopalan, K. V. (2003) Mechanistic and mutational studies of Escherichia coli molybdopterin synthase clarify the final step of molybdopterin biosynthesis J. Biol. Chem. 278, 14523-14532. PubMedID: 12571226
    52. Scheibner, K.A., Zhang, Z. and Cole, P.A. (2003) Merging fluorescence resonance energy transfer and expressed protein ligation to analyze protein-protein interactions Anal. Biochem.  317, 226-232. PubMedID: 12758261
    53. Zuberek, J., et al. (2003) Phosphorylation of eIF4E attenuates its interaction with mRNA 5' cap analogs by electrostatic repulsion: intein-mediated protein ligation strategy to obtain phosphorylated protein RNA 9, 52-61. PubMedID: 12554876
    54. Bonsager, B.C., et al. (2003) Purification and characterization of the beta-trefoil fold protein barley alpha-amylase/subtilisin inhibitor overexpressed in Escherichia coli Protein Expr. Purif. 2, PubMedID: 12880767
    55. David, R., et al. (2003) Semisynthesis and application of carboxyfluorescein-labelled biologically active human interleukin-8 Biol. Chem. 384, 1619-1630. PubMedID: 14719805
    56. Rak, A., et al. (2003) Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase Science 302, 646-50. PubMedID: 14576435
    57. Sinars, C.R., et al. (2003) Structure of the large FK506-binding protein FKBP51, an Hsp90-binding protein and a component of steroid receptor complexes Proc. Natl. Acad. Sci. USA 100, 868-873. PubMedID: 12538866
    58. Zhang, Z., et al. (2003) The role of C-terminal tyrosine phosphorylation in the regulation of SHP-1 explored via expressed protein ligation J. Biol. Chem. 278, 4668-4674. PubMedID: 12468540
    59. Grzybowska, B., Szweda, P. and Synowiecki, J. (2004) Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli: isolation and some properties of the enzyme Mol. Biotechnol. 26, 101-110. PubMedID: 14764935
    60. Durek T., Goody R.S and Alexandrov K. (2004) In vitro semisynthesis and applications of C-terminally modified Rab proteins Methods Mol Biol. 283, 233-244. PubMedID: 15197315
    61. Guo, C., et al. (2004) Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin Protein Expr Purif. 37, 361-367.
    62. Pezza, J.A., Allen, K.N. and Tolan, D.R. (2004) Intein-mediated purification of a recombinantly expressed peptide Chem Commun (Camb) 21, 2412-2413. PubMedID: 15514791
    63. Romanelli, A., et al. (2004) Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction Proc. Natl. Acad. Sci. USA 101, 6397-6402. PubMedID: 15087498
    64. Takeda, S.,et al. (2004) Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation Bioorg. Med. Chem. Lett. 14(10), 2407-10. PubMedID: 15109622
    65. Hemelaar, al. (2004) Specific and covalent targeting of conjugating and deconjugating enzymes of ubiquitin-like proteins Mol. Cell Biol. 24, 84-95. PubMedID: 14673145
    66. Durek, T., et al. (2004) Synthesis of fluorescently labeled mono- and diprenylated Rab7 GTPase J. Am. Chem. Soc. 126, 16368-16378. PubMedID: 15600338
    67. Lue, R.Y., et al. (2004) Versatile protein biotinylation strategies for potential high-throughput proteomics J Am. Chem. Soc. 126, 1055-1062. PubMedID: 14746473
    68. Sando, S., Kanatani, K., Sato, N., Matsumoto, H. Hohsaka, T. and Aoyama, Y. (2005) A small-molecule-based approach to sense codon-templated natural-unnatural hybrid peptides. Selective silencing and reassignment of the sense codon by orthogonal reacylation stalling at the single-codon level J. Am. Chem. Soc. 127, 7998-7999. PubMedID: 15926808
    69. Ingham, A.B., et al. (2005) A versatile system for the expression of nonmodified bacteriocins inEscherichia coli J. Appl. Microbiol. 98, 676-683. PubMedID: 15715871
    70. Colussi, P.A., Specht, C.A. and Taron, C.H. (2005) Characterization of a nucleus-encoded chitinase from the yeast Kluyveromyces lactis Appl. Environ. Microbiol. 71, 2862-2869. PubMedID: 15932978
    71. Ying, B.W., Taguchi, H., Kondo, M. and Ueda, T. (2005) Co-translational involvement of the chaperonin GroEL in the folding of newly translated polypeptides J. Biol. Chem. 280, 12035-12040. PubMedID: 15664980
    72. Kuruma, Y., Nishiyama, K., Shimizu, Y., Muller, M and Ueda, T. (2005) Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrance proteins Biotechnol, Prog. 21, 1243-1251. PubMedID: 16080708
    73. Rebets, Y., et al. (2005) DNA -binding activity of LndI protein and temporal expression of the gene that upregulates landomycin E production in Streptomyces globisporus 1912 Microbiology 281-290.
    74. Morassutti, C., et al. (2005) Expression of SMAP-29 cathelicidin-like peptide in bacterial cells by intein-mediated system Protein Expr. Purif. 39, 160-168.
    75. Colussi, P.A. and Taron, C.H. (2005) Kluyveromyces lactis LAC4 promoter variants that lack function in bacteria but retain full function in K. lactis Appl. Environ. Microbiol. 71, 7092-7098. PubMedID: 16269745
    76. Shimizu, Y. Kanamori, T. and Ueda, T. (2005) Protein synthesus by pure translation systems Methods 36, 299-304. PubMedID: 16076456
    77. Ge, X., et. al. (2005) Self-cleavable stimulus responsive tags for protein purification without chromatography J. Am. Chem. Soc 127, 11228-9. PubMedID: 16089436
    78. Girish, A., et al. (2005) Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing Bioorg. Med. Chem. Lett. 15, 2447-51. PubMedID: 15863295
    79. Goodin, J. L., et al. (2005) Yersinia pestis outer membrane type III secretion protein YscC: expression, purification, characterization, and induction of specific antiserum Protein Expr. Purif. 40, 152-163. PubMedID: 15721783
    80. Hackenberger, C. P., et. al. (2006) Expression of N-terminal Cys-protein fragments using an intein refolding strategy Bioorg. Med. Chem. 14, 5043-5048.
    81. Muto, H., Nakatogawa, H., and Ito, K. (2006) Genetically Encoded but Nonpolypeptide Prolyl-tRNA Functions in the A Site for SecM-Mediated Ribosomal Stall Mol. Cell. 22, 545-552. PubMedID: 16713584
    82. Van Ooyen, A.J.J., Dekker, P., Huang, M., Olsthoorn, M.M.A., Jacobs, D.I., Colussi P.A., and Taron, C.H. (2006) Heterologous protein production in the yeast Kluyveromyces lactis FEMS Yeast Res. 6, 381-392. PubMedID: 16630278
    83. Villemagne, D., Jackson, R. and Douthwaite, J.A. (2006) Highly efficient ribosome display selection by use of purified components for in vitro translation J. Immunol Methods 313, 140-148. PubMedID: 16730021
    84. Gottlieb, D., et al. (2006) Intein-mediated in vitro synthesis of lipidated Ras proteins Chem. Commun. (Camb) 21, 260-262. PubMedID: 16391727
    85. Wojtaszek, J., et al. (2006) LT CI, a novel chymotrypsin inhibitor of the potato I family from the earthworm Lumbricus terrestris. Purification, cDNA cloning, and expression Comp. Biochem. Physiol B. Biochem. Mol. Biol. 143, 465-472.
    86. Esipov, R.S., et. al. (2006) Production and purification of recombinant human glucagons overexpressed as intein fusion protein in Escherichia coli Protein Pept. Lett. 13, 343-347. PubMedID: 16712508
    87. Demidov, V.V., and Broude, N.E. (2006) Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro Nat. Protoc. 1, 714-719. PubMedID: 17406301
    88. Kalia, J., et al. (2006) Reactivity of intein thioesters: appending a functional group to a protein Chembiochem. 7, 1375-1383. PubMedID: 16897799
    89. Kaiser, C.M., Chang, H.C., Agashe, V.R., Lakshmipathy, S.K., Etchells, S.A., Hayer-Hartl, M., Hartl, F.U. and Barral, J.M. (2006) Real-time observation of trigger factor function on translating ribosomes Nature 444, 455-460. PubMedID: 17051157
    90. Eckenroth, B., et al. (2006) Semisynthesis and characterization of mammalian thioredoxin reductase Biochem. 45, 5158-70. PubMedID: 16618105
    91. Shimizu, Y. and Ueda, T. (2006) SmpB triggers GTP hydrolysis of elongation factor Tu on ribosome by compensating for the lack of codon-anticodon interaction during trans-translation initiation J. Biol. Chem. 281, 15987-15996. PubMedID: 16601123
    92. Lee, N.Y., et al. (2006) Structure and dynamics of the epidermal growth factor receptor C-terminal phosphorylation domain Protein Sci. 15, 1142-52. PubMedID: 16597832
    93. Grant, J., et al. (2006) The N terminus of GTP gamma S-activated transducin alpha-subunit interacts with the C terminus of the cGMP phosphodiesterase gammasubunit J. Biol. Chem.  281, 6194-6202. PubMedID: 16407279
    94. Stasser, J. P., et. al. (2007) A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase Biochem.  46, 11845-11856. PubMedID: 17902702
    95. Hassiepen, U., et. al. (2007) A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine110-glycine as substrate Anal Biochem. 371, 201-207. PubMedID: 17869210
    96. Sharma, C.M., Darfeuille, F., Plantinga, T.H., and Vogel, J. (2007) A small RNA regulates multiple ABC transporter mRNA's by targeting C/A-rich elements inside and upstream of ribosome-binding sites Gene Dev. 21, 2804-2817. PubMedID: 17974919
    97. Read, J.D., Colussi, P.A., Ganatra, M.B. and Taron, C.H. (2007) Acetamide selection of Kluyveromyces lactis cells transformed with an integrative vector leads to high frequency formation of multicopy strains Appl. Environ. Microbiol. 73, 5088-5096. PubMedID: 17586678
    98. Karow, A., et al. (2007) Authentic interdomain communication in an RNA helicase reconstituted by expressed protein ligation of two helicase domains FEBS J.  2, 463-473. PubMedID: 17229151
    99. Zhao, H.L., et al. (2007) Circumventing the heterogeneity and instability of human serum albumin-interferon-_2b fusion protein by altering its orientation J. Biotech.  131, 245-252. PubMedID: 17698234
    100. Sun, L., et al. (2007) Design, preparation and use of ligated phosphoproteins: A novel approach to study protein phosphatases by dot blot array, ELISA and Western blot assays Methods 42, 220-226. PubMedID: 17532508
    101. Hauser, P. S., et al. (2007) Expressed protein ligation using an N-terminal cysteine containing fragment generated in vivo from a pelB fusion protein Protein Expr. Purif. 54, 227-233. PubMedID: 17493830
    102. Wamalwa, B.M., et al. (2007) High-Level Heterologous Expression of Bacillus halodurans Putative Xylanase Xyn11A (BH0899) in Kluyveromyces lactis Biosci. Biotechnol. Biochem. 71, 688-693. PubMedID: 17341817
    103. Diao, H., et. al. (2007) Intein-mediated expression is an effective approach in the study of beta-defensins Biochem Biophys Res Commun 357, 840-846. PubMedID: 17445764
    104. Bhat, R. K. and Berger, S. (2007) New and easy strategy for cloning, expression, purification, and characterization of the 5S subunit of transcarboxylase from Propionibacterium f. shermanii Prep. Biochem. Biotechnol. 37, 13-26. PubMedID: 17134979
    105. Murtas, G., Kuruma, Y., Bianchini, P., Diaspro, A., and Luisi, P.L. (2007) Protein synthesis in liposomes with a minimal set enzymes Biochem. Biophys. Res. Commun.  363, 12-17. PubMedID: 17850764
    106. Ohta, A., Murakami, H., Higashimura, E., and Suga, H. (2007) Synthesis of Polyester by Means of Genetic Code Reprogramming Chem. Biol. 14, 1315-1322. PubMedID: 18096500
    107. Paulick, M. G., et al. (2007) Synthetic analogues of glycosylphosphatidylinositol-anchored proteins and their behavior in supported lipid bilayers J. Am. Chem. Soc. 129, 11543-11550. PubMedID: 17715922
    108. Kochinyan, S., et al. (2007) Use of intein-mediated phosphoprotein arrays to study substrate specificity of protein phosphatases Biotechniques 42, 63-69. PubMedID: 17269486
    109. Ottesen, J. et al. (2008) An amalgamation of solid phase peptide synthesis and ribosomal peptide synthesis Biopolymers 90, 406-414. PubMedID: 17636509
    110. Zhao, W. et al. (2008) An efficient on-column expressed protein ligation strategy: application to segmental triple labeling of human apolipoprotein E3 Protein Sci.  17, 736-747. PubMedID: 18305193
    111. Kazuta, Y., Adachi, J., Matsuura, T., Ono, N., Mori, H. and Yomo T. (2008) Comprehensive Analysis of the Effects of Eschericia coli ORF's on Protein Translation Reaction Molecular & Cellular Proteomics 7, 1530-1540. PubMedID: 18453339
    112. Babu, S.K., et. al. (2008) Construction of intein mediated hGMCSF expression vector and its prufication in Pichia pastoris Protein Exp. Purif. 57, 201-205. PubMedID: 18309571
    113. Chen, Y. Q., et. al. (2008) Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners Protein Exp. Purif. 57, 303-311. PubMedID: 17977015
    114. Platko, J.D., et al. (2008) Heterologous expression of Mytilus californianus foot protein three (Mcfp-3) in Kluyveromyces lactis Platko, J.D., et al. 57, 57-62. PubMedID: 17923416
    115. Skrisovska, L. et al. (2008) Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L. J. Mol. Biol. 375, 151-164. PubMedID: 17936301
    116. Vazquez-Laslop, N., Thum, C., and Mankin, A.S. (2008) Molecular Mechanism of Drug-Dependent Ribosome Stalling  Mol. Cell. 30, 190-202. PubMedID: 18439898
    117. Swaim, C.L., et al. (2008) Physical and computational analysis of the yeast Kluyveromyces lactissecreted proteome Proteomics 8, 2714-2723. PubMedID: 18601269
    118. Zhao, Z., et. al. (2008) Purification of green fluorescent protein using a two-intein system Appl. Microbiol. Biotechnol. 77, 1175-1180. PubMedID: 17973109
    119. Subtelny. A. O.,Hartman, M. C. T., and Szostak, J. W. (2008) Ribosomal Synthesis of N-Methyl Peptides  J. Am. Chem. Soc. 130, 6131-6136. PubMedID: 18402453
    120. Sako, Y., Goto, Y., Murakami, H., and Suga, H. (2008) Ribosomal Synthesis of Peptidase-Resistant Peptides Closed by a Nonreducible Inter-Side-Chain Bond ACS Chem. Biol.  3, 241-249. PubMedID: 18338852
    121. Takakuwa, N., Oshnishi, M., and Oda, Y. (2008) Significance of the KLAC1 gene in glucosylceramide production by Kluyveromyces lactis FEMS Yeast Res 9, 839-846. PubMedID: 18631186
    122. Uemura, S., Iizuka, R., Ueno, T., Shimizu, Y., Taguchi, H., Ueda, T., Puglisi. J., and Funatsu, T. (2008) Single molecule imaging of full protein synthesis by immobilized ribosomes Nuc. Acid. Res.  36, e90. PubMedID: 18511463
    123. Sugiki, T. Shimada, I. and Takahashi H. (2008) Stable isotope labeling of protein byKluyveromyces lactis for NMR study J. Biomol NMR. 42, 159-62. PubMedID: 18827973
    124. Foster, J.M., Raverdy, S., Ganatra, M.B., Colussi, P.A., Taron, C.H. and Carlow, C.K.S. (2008) The Wolbachia endosymbiont of Brugia malayi has an active phosphoglycerate mutase: a candidate target for anti-filarial therapies Parasitol. Res.  PubMedID: 19043737
    125. Feng, Y. and Cronan, J. E. (2009) A new member of the Eschericia coli fad regulon: transcriptional regulation of fadM (ybaW) J. Bacteriol. 191, 6320-6328. PubMedID: 19684132
    126. Feng, Z., et al (2009) Effect of temperature-shift strategy on recombinat chymosin of Kluyveromyces Lactis Heilongjiang Animal Science and Veterinary Medicine 2009-2013.
    127. Tanner, D., Cariello, D., Woolstenhulme, C., Broadbent, M. and Buskirk, A. (2009) Genetic identification of nascent peptides that induce ribosome stalling J. Biol. Chem. 284, 34809-34818. PubMedID: 19840930
    128. Solaroli, N., Panayiotou, C., Johansson, M., and Karlsson, A. (2009) Identification of two active functional domains of human adenylate kinase 5 FEBS Lett. 583, 2872-2876. PubMedID: 19647735
    129. Talabot-Ayer, D., Lamacchia, C., Gabay, C., and Palmer, G. (2009) Interleukin-33 is biologically active independently of Caspase-1 cleavage J. Biol. Chem. 284, 19420-19426. PubMedID: 19465481
    130. Estevez-Torres, A., Crozatier, C., Diguet, A., Hara, T., Saito, H., Yoshikawa, K., and Baigl, D. (2009) Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder Proc. Natl. Acad. Sci.  106, 12219-12223. PubMedID: 19617550
    131. Chattopadhaya, S., et al. (2009) Site-specific covalent labeling of proteins inside live cells using small molecule probes Bioorg. Med. Chem. 17, 981-9. PubMedID: 18261914
    132. Che, N., et. al. (2009) Soluble expression and one-step purification of a neurotoxin Huwentoxin-I in Escherichia coli Protein Expr Purif. 65, 154-159. PubMedID: 19217942
    133. Madinger, C.L., Sharma, S.S., Anton, B.P., Fields, L.G., Cushing, M.L., Canovas, J., Taron, C.H. and Benner, J.S. (2009) The effect of carbon source on the secretome of Kluyveromyces lactis Proteomics 9, 4744-4754. PubMedID: 19743416
    134. Chattopadhaya, S., et al. (2009) Use of intein-mediated protein ligation strategies for the fabrication of functional protein arrays Methods Enzymol. 462, 195-223. PubMedID: 19632476
    135. Hondal, R. J. (2009) Using chemical approaches to study selenoproteins-Focus on thioredoxin reductases Biochim. Biophys. Acta. 1790, 1501-1512. PubMedID: 19406205
    136. Zheng, Y., Posfai, J., Morgan, R. D., Vincze, T., and Roberts, R.J. (2009) Using shotgun sequence data to find active restriction enzyme genes Nuc. Acid. Res. 37, el. PubMedID: 18988632
    137. Karukurichi, K. et al. (2010) Analysis of p300/CBP histone acetyltransferase regulation using circular permutation and semisynthesis J. Am. Chem. Soc. 132, 1222- 1223. PubMedID: 20063892
    138. Chen, J., et al. (2010) Chemically ubiquitylated PCNA as a probe for eukaryotic translesion DNA synthesis Nat. Chem. Biol. 6, 270-272. PubMedID: 20208521
    139. Collins, E. D., et. al. (2010) Cloning the human vitamin D receptor into the pTwin-1 expression vector J Steroid Biochem Mol Biol PubMedID: 20171280
    140. Feng, Z., et al. (2010) Codon optimization of the calf prochymosin gene and its expression in Kluyveromyces lactis World J. Microbiol. Biotechnol. 26, 895-901.
    141. Lu, X., et al. (2010) Designed semisynthetic protein inhibitors of ub/ubl e1 activating enzymes J. Am. Chem. Soc. 132, 1748-1749. PubMedID: 20099854
    142. Kuo, D., et al. (2010) Evolutionary divergence in the fungal response to fluconazole revealed by soft clustering Gen. Bio.  11, R77. PubMedID: 20653936
    143. Yu, H. H., et. al. (2010) Expressed protein ligation for the preparation of fusion proteins with cell penetrating peptides for endotoxin removal and intracellular delivery Biochim Biophys Acta PubMedID: 20170629
    144. Manconi, B., et. al. (2010) Expression, purification, phosphorylation and characterization of recombinant human statherin Protein Expr Purif. 69, 219-225.
    145. Yuan W, et al. (2010) Gene synthesis of the bovine prochymosin gene and high-level expression in Kluyvermyces lactis Sheng Wu Gong Cheng Xue Bao 26, 1281-1286. PubMedID: 21141120
    146. Rocha, S.N., et al. (2010) Heterologous expression of glucose oxidase in the yeastKluyveromyces marxianus Microb. Cell Fact. 21, PubMedID: 20092622
    147. Asahara, H. and Chong, S. (2010) In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme Nucleic Acids Res. 38, e141. PubMedID: 20457746
    148. Lu, M., et. al. (2010) Purification of untagged HIV-1 reverse transcriptase by affinity chromatography Protein Expr. Purif. 71, 231-239. PubMedID: 20060474
    149. Ueda, T., Kanamori, T., Ohashi, H. (2010) Ribosome display with the PURE technology Methods Mol Biol. 607, 219-25. PubMedID: 20204860
    150. Theerthagiri, G., Eisenhardt, N., Schwarz, H. and Antonin, W. (2010) The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex  J. Cell Biol.  189, 1129-1142. PubMedID: 20566687
    151. Noto, T., Kurth, H., Kataoka, K., Aronica, L., DeSouza, L., Siu, K., Pearlman, R., Gorovsky, M. and Mochizuki, K. (2010) The tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus Cell 140, 692-703. PubMedID: 20211138
    152. Shen, B.W., Heiter, D.F., Chan, S.H., Wang, H., Xu, S.Y., Morgan, R.D., Wilson, G.G. and Stoddard, B.L. (2010) Unusual target site disruption by the rare-cutting HNH restriction endonuclease PacI Structure 18, 734-743. PubMedID: 20541511
    153. Ganatra, M.B., Rainauskas, S., Hong, J.M., Taylor, T.E., Denson, J.P.M., Esposito, D., Read, J.D., Schmeisser, H., Zoon, K.C., Hartley, J.L. and Taron, C.H. (2011) A set of aspartyl protease-deficient strains for improved expression of heterologous proteins in Kluyveromyces lactis FEMS Yeast Res. 11, 168-178. PubMedID: 21166768
    154. Feng, Z., et al. (2011) Disruption of PMR1 in Kluyveromyces lactis improves secretion of calf prochymosin J. Sci. Food Agric. 15, 100-103.
    155. Narayan, V., Pion, E., Landré, V., Müller, P. and Ball, K.L. (2011) Docking-dependent ubiquitination of the interferon regulatory factor-1 tumor suppressor protein by the ubiquitin ligase CHIP J. Biol. Chem. 286, 607-619. PubMedID: 20947504
    156. Rosner, K., Kasprzak, M.F., Horenstein, A.C., Thurston, H.L., Abrams, J., Kerwin, L.Y., Mehregan, D.A. and Mehregan, D.R. (2011) Engineering a waste management enzyme to overcome cancer resistance to apoptosis: adding DNase1 to the anti-cancer toolbox Cancer Gene Ther. 18, 346-357. PubMedID: 21233855
    157. Zhou, X., et al. (2011) Expression of heparin sulfate sulfotransferases in Kluyveromyces lactisand preparation of PAPS Glycobiology Advance Access
    158. Rocha S.N., et al. (2011) Heterologous expression of a thermophilic esterase in Kluyveromyces yeasts Appl. Microbiol. Biotechnol. 89, 375-85. PubMedID: 20862582
    159. Yamamoto, S., Izumiya, H., Mitobe, J., Morita, M., Arakawa, E., Ohnishi, M. and Watanabe, H. (2011) Identification of a chitin-induced small RNA that regulates translation of the tfoX gene, encoding a positive regulator of natural competence in Vibrio cholerae J Bacteriol. 193, 1953-1965. PubMedID: 21317321
    160. Iizuka, R., Yamagishi-Shirasaki, M. and Funatsu, T. (2011) Kinetic study of de novo chromophore maturation of fluorescent proteins Anal. Biochem. Apr 1., PubMedID: 21459075
    161. Midon, M., Schäfer, P., Pingoud, A., Ghosh, M., Moon, A.F., Cuneo, M.J., London, R.E. and Meiss, G. (2011) Mutational and biochemical analysis of the DNA-entry nuclease EndA from Streptococcus pneumoniae Nucleic Acids Res. 39, 623-634. PubMedID: 20846957
    162. Feng, Z., et al. (2011) Optimization of Medium Composition for Production of Recombinant Calf Chymosin from Kluyveromyces lactis in Submerged Fermentation J. of Northeast Agricultural University 18, 56-62.
    163. Lamichhane, T.N., Abeydeera, N.D., Duc, A.C., Cunningham, P.R. and Chow, C.S (2011) Selection of peptides targeting helix 31 of bacterial 16S ribosomal RNA by screening M13 phage-display libraries Molecules 16, 1211-1239. PubMedID: 21278676
    164. Panayiotou, C., Solaroli, N., Xu, Y., Johansson, M. and Karlsson, A. (2011) The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes Biochem J.  2433, 527-534. PubMedID: 21080915
    165. De Masi, F., Grove, C.A., Vedenko, A., Alibés, A., Gisselbrecht, S.S., Serrano, L., Bulyk, M.L. and Walhout, A.J. (2011) Using a structural and logics systems approach to infer bHLH-DNA binding specificity determinants Nucleic Acids Res. 18-Feb, PubMedID: 21335608
    166. Handa, Y., Inaho, N. and Nameki, N. (2011) YaeJ is a novel ribosome-associated protein inEscherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes Nucleic Acids Res.  39, 1739-1748. PubMedID: 21051357

    Protein Expression and Purification: Selection Chart

    Each NEB expression technology offers different advantages which enable you to choose the strategy that best suits your protein expression and purification needs.