New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.
SHuffle cells can be an alternative expression host for proteins that fail to express in common protein expression strains, such as BL21(DE3). This characteristic of SHuffle cells was demonstrated by the expression of a polymerase, which otherwise failed to be expressed in E. coli B and K12 strains. The polymerase lacks any cysteine, and its folding is therefore unrelated to the redox properties of SHuffle cells.
Learn about SHuffle® strains for the expression of multi-disulfide bonded and difficult-to-express proteins