
pMAL™
Choose Type:
- Cleavage of the Fusion Protein Generated Using The pMAL Protein Fusion and Purification System (E8200)
- Cloning a PCR Fragment Into a pMAL Expression Vector (E8200)
- Isolation of MBP-fusion protein using Anti-MBP Magnetic Beads
- Isolation of MBP-fusion protein using Amylose Magnetic Beads
- Purification of a Fusion Protein generated by The pMAL Protein Fusion and Purification System (E8200)
- Separating the Protein of Interest from MBP after Protease Cleavage Using The pMAL Protein Fusion and Purification System (E8200)
- Western Transfer Protocol for Anti-MBP Monoclonal Antibody
- Western Transfer Protocol for Anti-MBP Monoclonal Antibody, HRP Conjugated
- Quick Start Guide (E8200)
- Protein Expression with T7 Express strains
- Expression Using SHuffle®
-
Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
- Competent Cell Brochure
- Protein Expression & Purification Brochure
- Competent Cell Product Comparison
- Protein Expression and Purification Selection Chart
- Convenient Formats of Competent Cells
Feature Articles
Brochures
Selection Tools
Usage Guidelines
- Reliable E. coli expression – Substantial yields (up to 100 mg/L) in more than 75% of the cases tested
- Enhances solubility – Proteins that are insoluble when expressed in E. coli unfused are often soluble when fused to MBP (1).
- Expression in either the cytoplasm or periplasm – Periplasmic expression enhances folding of protein with disulfide bond.
References
- Kapust, R.B. and Waugh, D.S. (1999) Prot. Sci., 8, 1668–1674. PMID: 10452611

Lane 1: Protein Ladder (NEB #P7703).
Lane 2: uninduced cells.
Lane 3: induced cells.
Lane 4: purified protein eluted from amylose column with maltose.
Lane 5: purified protein after Factor Xa cleavage.
Lane 6: paramyosin fragment in flow-through from second amylose column.
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.