
IMPACT™
Choose Type:
- Affinity Purification and On-column Cleavage (E6901)
- Construction of the Fusion Plasmid (E6901)
- Fusion Protein Expression (E6901)
- Isolation of CBD-fusion protein using Chitin Magnetic Beads
- Isolation of MBP-fusion protein using Amylose Magnetic Beads
- Preparation of Media and Solutions (E6901)
- Primer Design for Restriction Enzyme Cloning (E6901)
- Simplified Expression and Purification Protocol (E6901)
- Protein Expression with T7 Express strains
- Expression Using SHuffle®
- Fusion Constructs (E6901)
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Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
- Competent Cell Brochure
- Protein Expression & Purification Brochure
- Competent Cell Product Comparison
- IMPACT™ Vectors and Applications
- Protein Expression and Purification Selection Chart
Feature Articles
Brochures
Selection Tools
- Yields proteins with native sequence
- One-step affinity purification with no additional steps to remove affinity tag
- Release from the fusion partner without the use of proteases
- Fusion to either the C-terminus or N-terminus of the target protein
- The ability to label the C-terminus of the target protein
- T7 Promoter for higher levels of expression and tight transcriptional control
- The ability to introduce non-coded amino acids, fatty acids or chemically modified amino acid residues (segmental labeling of proteins for NMR analysis)

Lane 2: induced cell extract showing expressed fusion protein.
Lane 3: MBP fractions eluted after inducing cleavage overnight at 4°C.
Lane 4: MBP ligated to a peptide containing an N-terminal cysteine. Marker M is the Protein Ladder (NEB #P7703)
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