A rapid method for gene expression analysis, PURExpress® is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation.
PURExpress® is a registered trademark of New England Biolabs, Inc.
- Analysis of Synthesized Protein using PURExpress (E6800)
- Analysis of Synthesized Protein using PURExpress (E3313)
- Analysis of Synthesized Protein using PURExpress (E6840)
- Determination of Protein Synthesis Yield with PURExpress (E3313)
- Determination of Protein Synthesis Yield with PURExpress (E6800)
- Determination of Protein Synthesis Yield with PURExpress (E6840)
- Measurement of 35S-Methionine Incorporation by TCA Precipitation and Yield Determination using PURExpress
- Protein Synthesis Reaction using PURExpress (E3313)
- Protein Synthesis Reaction using PURExpress (E6800)
- Protein Synthesis Reaction using PURExpress® ∆ (aa, tRNA) Kit (E6840)
- PURExpress Disulfide Bond Enhancer (E6820)
- Purification of Synthesized Protein using Reverse His-tag Purification
- Analysis of Synthesized Protein using PURExpress (E6850)
- Protein Synthesis Reaction using PURExpress® ∆ RF123 Kit (E6850)
- Determination of Protein Synthesis Yield with PURExpress (E6850)
- Protocol for Avoiding Rnase Contamination using Murine Rnase Inhibitor (M0314)
- Poly(A) Tailing of RNA using E. coli Poly(A) Polymerase (NEB# M0276)
- NEBExpress® Cell-free E. coli Protein Synthesis System
- Use of the PURExpress® in vitro Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle® Competent E. coli for heterologous in vitro and in vivo cellulase expression.
- Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants
The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
- Protein Expression & Purification Brochure
- Protein Expression and Purification Selection Chart
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- His-Tagged Translation Factors
- Initiation Factors (IF1, IF2, IF3)
- Elongation Factors (EF-Tu, EF-Ts, EF-G)
- Release Factors (RF1, RF2, RF3)
- Ribosome Recycling Factor
- 20 Aminoacyl tRNA synthetases
- Methionyl tRNA formyltransferase
- E. coli Ribosomes
- E. coli tRNAs
- Energy Regeneration System
- NTPs, Amino Acids, Salts, Buffer
In addition, recombinant T7 RNA polymerase is used to couple transcription to translation. The PURE system represents an important step towards a totally defined in vitro transcription/translation system, thus avoiding the “black box” nature of the cell extract-based systems.
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Watch this tutorial explaining the streamlined workflow for our new NEBExpress® Cell-free Protein Synthesis System to learn how you can easily synthesize your protein in as little as 2 to 4 hours.
NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.