RT-PCR
OneTaq® One-Step RT-PCR Kit
The OneTaq One-Step RT-PCR Kit offers sensitive and robust end-point detection of RNA templates. cDNA synthesis and PCR amplification steps are performed in a single reaction using gene-specific primers, resulting in a streamlined RT-PCR protocol. The kit combines three optimized mixes: OneTaq One-Step Enzyme Mix, OneTaq One-Step Reaction Mix and OneTaq One-Step Quick-Load Reaction Mix. The Enzyme Mix combines ProtoScript II Reverse Transcriptase, Murine RNase Inhibitor and OneTaq Hot Start DNA Polymerase.
OneTaq® RT-PCR Kit
The OneTaq® RT-PCR Kit (NEB #E5310) combines two powerful mixes, M-MuLV Enzyme Mix and OneTaq Hot Start 2X Master Mix with Standard Buffer for 2-step RT-PCR applications. The two mixes require minimal handling during reaction setup and yet offer consistent and robust RT-PCR reactions.
ProtoScript® and OneTaq® are registered trademarks of New England Biolabs, Inc.
Choose Type:
Protocols for RT-PCR
- First Strand cDNA Synthesis Protocols (E6560)
- Reaction Conditions (E5315)
- Standard PCR Protocol (E5315)
- One-Step RT-PCR Protocols (E5315)
- Typical RT-LAMP Protocol
- Typical cDNA Synthesis Protocol
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0253)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0368)
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0368)
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0253)
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0368)
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0253)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0277)
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0277)
- First Strand cDNA Synthesis Protocols (E6300)
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0277)
- PCR Amplification with OneTaq® RT-PCR Kit
- First strand cDNA synthesis OneTaq® RT-PCR Kit
