RT-PCR
OneTaq® One-Step RT-PCR Kit
The OneTaq One-Step RT-PCR Kit offers sensitive and robust end-point detection of RNA templates. cDNA synthesis and PCR amplification steps are performed in a single reaction using gene-specific primers, resulting in a streamlined RT-PCR protocol. The kit combines three optimized mixes: OneTaq One-Step Enzyme Mix, OneTaq One-Step Reaction Mix and OneTaq One-Step Quick-Load Reaction Mix. The Enzyme Mix combines ProtoScript II Reverse Transcriptase, Murine RNase Inhibitor and OneTaq Hot Start DNA Polymerase.
OneTaq® RT-PCR Kit
The OneTaq® RT-PCR Kit (NEB #E5310) combines two powerful mixes, M-MuLV Enzyme Mix and OneTaq Hot Start 2X Master Mix with Standard Buffer for 2-step RT-PCR applications. The two mixes require minimal handling during reaction setup and yet offer consistent and robust RT-PCR reactions.
ProtoScript® and OneTaq® are registered trademarks of New England Biolabs, Inc.
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Protocols for RT-PCR
- Reaction Conditions (E5315)
- Standard PCR Protocol (E5315)
- One-Step RT-PCR Protocols (E5315)
- Typical RT-LAMP Protocol
- Typical cDNA Synthesis Protocol
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0277)
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0277)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0253)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0368)
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0368)
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0253)
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0253)
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0368)
- First Strand cDNA Synthesis Protocols (E6300)
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0277)
- PCR Amplification with OneTaq® RT-PCR Kit
- First strand cDNA synthesis OneTaq® RT-PCR Kit
- First Strand cDNA Synthesis Protocols (E6560)
Legal Information
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
