Q5® High-Fidelity DNA Polymerases
Fidelity at its finest – for over 10 years.
Q5® High-Fidelity DNA Polymerase (NEB #M0491) sets a new standard for both fidelity and robust performance. With fidelity >280 times higher than Taq, Q5 results in ultra-low error rates. Q5 is a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Q5 is also available in a hot start format (NEB #M0493). In contrast to chemically modified or antibody-based hot start polymerases, Q5 Hot Start High-Fidelity DNA Polymerase utilizes engineered oligonucleotides known as aptamers. The aptamer binds to the polymerase through non-covalent interactions, blocking enzyme activity during the reaction setup, and is dissociated during normal cycling conditions. Reactions can be set up at room temperature and a separate high temperature activation step is not required, shortening reaction times.
Q5 master mixes (NEB #M0492, #M0494) contain dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification, regardless of GC content.
Q5U® Hot Start High-Fidelity DNA Polymerase (NEB #M0515) is a modified version of Q5 High-Fidelity DNA Polymerase containing a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases. This is useful for amplifying bisulfite-converted, enzymatically-deaminated, or damaged DNA, preventing carryover contamination in PCR (when used with dUTP and UDG), and in USER cloning methods. Learn more about this product.
The LunaScript® Multiplex One-Step RT-PCR Kit (NEB #E1555) combines Q5 with the thermostable Luna® WarmStart® Reverse Transcriptase for a streamlined one-step RT-PCR protocol with superior multiplexing and sensitivity down to 0.01 pg of human total RNA. Learn more about this product.
NEW: Q5 Blood Direct 2X Master Mix (NEB #M0500) can amplify a wide variety of targets direct from dried blood spots or up to 30% whole human blood, skipping DNA purification. Learn more about this product.
Choose Type:
- PCR Using NEBNext® High-Fidelity 2X PCR Master Mix (M0541)
- PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (M0493)
- Protocol for Q5® Hot Start High-Fidelity 2X Master Mix
- Protocol for Q5® High-Fidelity 2X Master Mix
- PCR Optimization (E0555)
- Protocol for a Routine PCR (E0555)
- PCR Using Q5® High-Fidelity DNA Polymerase (M0491)
- Protocol for a PCR reaction using NEBNext® Q5® Hot Start HiFi PCR Master Mix (M0543)
- Protocol for a PCR reaction using NEBNext® Ultra™ II Q5® Master Mix (M0544)
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Polymerase Fidelity: What is it, and what does it mean for your PCR?
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Using aptamers to control enzyme activity: Hot Start Taq and beyond
This article describes the use of engineered oligonucleotides known as aptamers in regulating the enzyme activity of polymerases and reverse transcriptases.
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Understanding Variability in DNA Amplification Reactions
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Anatomy of a Polymerase - How Function and Structure are Related
Read about the relationship between Polymerase structure and function when copying DNA.
- PCR Reagents Brochure
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
Feature Articles
Brochures
Selection Tools
Troubleshooting Guides
Usage Guidelines
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