Deep Vent® (exo-) DNA Polymerase
Deep Vent (exo-) DNA Polymerase has been genetically engineered to eliminate the 3′→ 5′ proofreading exonuclease activity associated with Deep Vent DNA Polymerase. Deep Vent (exo-) DNA Polymerase is even more stable than Vent® (exo-) DNA Polymerase with a half-life of 23 hours at 95°C and 8 hours at 100°C. Both Deep Vent (exo-) and Vent (exo-) DNA Polymerase are suitable for primer extensions and high temperature (72°C) DNA sequencing.
Deep Vent® DNA Polymerase
Deep Vent DNA Polymerase is the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3′→ 5′ proofreading exonuclease activity. Deep Vent is even more stable than Vent at temperatures of 95 to 100°C.
Vent® (exo-) DNA Polymerase
Vent (exo-) DNA Polymerase has been genetically engineered to eliminate the 3′→ 5′ proofreading exonuclease activity associated with Vent DNA Polymerase (1). This is the preferred form for high-temperature dideoxy sequencing reactions and for high yield primer extension reactions. The fidelity of polymerization by this form is reduced to a level about 2-fold higher than that of Taq DNA Polymerase (2,3).
Vent® DNA Polymerase
Vent DNA Polymerase is a high-fidelity thermophilic DNA polymerase. The fidelity of Vent DNA Polymerase is 5-15-fold higher than that observed for Taq DNA Polymerase (1,2). This high fidelity derives in part from an integral 3′→5′ proofreading exonuclease activity in Vent DNA Polymerase (1,3). Greater than 90% of the polymerase activity remains following a 1 hour incubation at 95°C.
Therminator™ DNA Polymerase
Therminator DNA Polymerase is a 9°N™ DNA Polymerase variant with an enhanced ability to incorporate modified substrates such as dideoxynucleotides, ribonucleotides and acyclonucleotides (1,2).
- Protocol for a Routine Vent PCR
- Guidelines for PCR Optimization for Vent DNA Polymerase
- PCR Protocol for Hemo KlenTaq®
- Protocol for a Routine Vent (exo-) PCR
- Protocol for a Routine Deep Vent® PCR
- Guidelines for PCR Optimization for Deep Vent® DNA Polymerase
- Protocol for a Routine Deep Vent® (exo-) PCR
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.