New England Biolabs supplies a selection of highly pure nucleotides for use with DNA and RNA polymerases.
Deoxynucleotide Solution Set (NEB #N0446)
The Deoxynucleotide Solution Set contains four separate 100 mM solutions of ultrapure nucleotides (dATP, dCTP, dGTP, and dTTP).
Deoxynucleotide Solution Mix (NEB #N0447)
The Deoxynucleotide Solution Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP. Each nucleotide is present at a concentration of 10 mM in the mixture for a total dNTP concentration of 40 mM.
7-deaza-dGTP* (NEB #N0445)
7-deaza-dGTP is a useful additive for the PCR of GC-rich templates; contains a 5 mM solution of 7-deaza-dGTP as a dilithium salt.
* licensed from Roche Diagnostics GmbH
Acyclonucleotide Set (NEB #N0460)
The Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP), supplied as a dry powder.
dATP Solution (NEB #N0440)
The dATP solution contains 0.25 ml of 100 mM ultrapure dATP.
Ribonucleotide Solution Set (NEB #N0450)
Ribonucleotide Solution Set consists of four separate 100 mM solutions of ATP, GTP, CTP and UTP.
Ribonucleotide Solution Mix (NEB #N0466)
The Ribonucleotide Solution Mix is an equimolar mixture of ribonucleotide triphosphates (rATP, rCTP, rGTP and rUTP). Each is supplied at a concentration of 80 mM for a total concentration of 320 mM.
- How much is in each vial of the Deoxynucleotide Solution Set?
- What is supplied with the Deoxynucleotide Solution Set?
- How does this product differ from the Deoxynucleotide Solutions Set (NEB# N0446)?
- What is supplied with the Deoxynucleotide Solution Mix?
- What is in the Deoxynucleotide Solution Mix?
- What is 7-deaza-dGTP used for?
- Polymerases and Amplification FAQs
Anatomy of a Polymerase - How Function and Structure are Related
Read about the relationship between Polymerase structure and function when copying DNA.
DNA Damage - the major cause of missing pieces from the DNA puzzle
Understanding Variability in DNA Amplification Reactions
- PCR Brochure
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- DNA Damage and PreCR
- Guidelines for PCR Optimization with Taq DNA Polymerase
Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template?
Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end?
Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable?
Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.