New England Biolabs supplies a selection of highly pure nucleotides for use with DNA and RNA polymerases.
Deoxynucleotide Solution Set (NEB #N0446)
The Deoxynucleotide Solution Set contains four separate 100 mM solutions of ultrapure nucleotides (dATP, dCTP, dGTP, and dTTP).
Deoxynucleotide Solution Mix (NEB #N0447)
The Deoxynucleotide Solution Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP. Each nucleotide is present at a concentration of 10 mM in the mixture for a total dNTP concentration of 40 mM.
7-deaza-dGTP* (NEB #N0445)
7-deaza-dGTP is a useful additive for the PCR of GC-rich templates; contains a 5 mM solution of 7-deaza-dGTP as a dilithium salt.
* licensed from Roche Diagnostics GmbH
Acyclonucleotide Set (NEB #N0460)
The Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP), supplied as a dry powder.
dATP Solution (NEB #N0440)
The dATP solution contains 0.25 ml of 100 mM ultrapure dATP.
Ribonucleotide Solution Set (NEB #N0450)
Ribonucleotide Solution Set consists of four separate 100 mM solutions of ATP, GTP, CTP and UTP.
Ribonucleotide Solution Mix (NEB #N0466)
The Ribonucleotide Solution Mix is an equimolar mixture of ribonucleotide triphosphates (rATP, rCTP, rGTP and rUTP). Each is supplied at a concentration of 80 mM for a total concentration of 320 mM.
FAQs for Nucleotide Solutions
- What is supplied with the Deoxynucleotide Solution Set?
- How much is in each vial of the Deoxynucleotide Solution Set?
- What is 7-deaza-dGTP used for?
- How does this product differ from the Deoxynucleotide Solutions Set (NEB# N0446)?
- What is supplied with the Deoxynucleotide Solution Mix?
- What is in the Deoxynucleotide Solution Mix?
- Polymerases and Amplification FAQs
Protocols for Nucleotide Solutions
Anatomy of a Polymerase - How Structure Effects Function
DNA Damage - the major cause of missing pieces from the DNA puzzle
Understanding Variability in DNA Amplification Reactions
The PCR brochure provides product information on the wide range of DNA polymerases available from NEB, including tools for selection and troubleshooting tips.
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- DNA Damage and PreCR
- Guidelines for PCR Optimization with Taq DNA Polymerase
Other Tools & Resources
DNA Polymerase Selection Chart
Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template?
Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end?
Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable?
Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?