Taq DNA Polymerases

Polymerases and Amplification Technologies
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    Taq DNA Polymerase is the industry standard for routine PCR. Taq with Thermopol Buffer (NEB #M0267) is available in economical extra-large pack sizes. NEB provides high quality recombinant Taq at an exceptional value. Taq is available with different reaction buffers to accommodate a variety of PCR applications. For example, standard Taq Buffer is designed to support existing PCR platforms and is an ideal choice for DHPLC and high-throughput applications, while ThermoPol™ Buffer is formulated to promote high product yields, even under demanding conditions. Hot Start Taq formulations (NEB #M0495, NEB #M0196) are also available for flexibility during reaction setup and increased specificity.


    Taq DNA Polymerase is available in several convenient formats. The Taq PCR kit (NEB #E5000) includes all the reagents for 200 reactions. Taq 2X (NEB #M0270) or 5X (NEB #M0285) Master Mixes are ideal for high-throughput applications. Quick-Load® Taq 2X Master Mix (NEB #M0271) includes dye for direct gel loading.

    For exceptional performance across a wide range of templates, try OneTaq or OneTaq Hot Start.

    ThermoPol™ is a trademark of New England Biolabs, Inc.
    Quick-Load® is a register trademark of New England Biolabs, Inc.

    1. Overview of PCR

      This overview will walk you through how the Polymerase Chain Reaction (PCR) works.

    DNA Polymerase Selection Chart

    NEB offers a guidelines for choosing the correct DNA polymerase for your application by providing a list of specific properites.
    Several factors govern which polymerase should be used in a given application, including: 

    Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template? 

    Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end? 

    Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable? 

    Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?

    PCR Selection Tool

    Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme technology and bring unparalleled confidence to your experiments.

    Taq Buffer Selection Chart