DNA polymerases with high fidelity are important for applications in which the DNA sequence needs to be correct after amplification. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion® High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Reagent selection includes a standalone enzyme, master mix and kit format, as well as a choice of reaction buffers for amplification of difficult templates. Phusion Hot Start Flex DNA Polymerase is available as standalone enzyme or in a master mix format and enables high specificity amplification of a broad range of templates. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons.
Thermo Scientific® is a registered trademark of Thermo Fisher Scientific.
Phusion® was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.