OneTaq® DNA Polymerases

  • My NEB
  • Print
  • PDF
  • See what our customers are saying about OneTaq® DNA Polymerases

    An optimized blend of Taq and Deep VentR™ DNA polymerases, OneTaq® and OneTaq® Hot Start DNA Polymerases offer robust amplification across a wide range of templates. The 3´–5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robustness of Taq, and the hot start formulation combines convenience with decreased interference from primer dimers and secondary products. Additionally, OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template's GC content. Both polymerases are available in master mix and Quick-Load® master mix formats.

    In contrast to chemically modified or antibody-based hot start polymerases, NEB's OneTaq Hot Start utilizes aptamer technology. This unique modified oligonucelotide binds to the polymerase through non-covalent interactions, blocking polymerase activity at temperatures below 45°C. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature.

    • How to Amplify GC-rich DNA

      Looking for tips on dealing with GC-bias in DNA amplification? NEB scientists have the expertise you need!

      scroll to see additional videos
    • Choose the Right DNA Polymerase for PCR

      Make sure you're using the optimal polymerase for your DNA amplifications. Get tips on choosing the right DNA Polymerase for your application.

      scroll to see additional videos

    OneTaq Hot Start DNA Polymerase

    Amplification of a selection of sequences with varying GC content from human and C. elegans genomic DNA using OneTaq Hot Start DNA Polymerase. The presence or absence of an extended room temperature incubation does not affect performance. GC content is indicated above gel. Marker M is the 1 kb DNA Ladder (NEB# N3232).

    Deep VentR™ is a trademark of New England Biolabs, Inc.
    OneTaq®, LongAmp® and Quick-Load® are registered trademarks of New England Biolabs, Inc.

    Advantages

    • Exceptional performance in endpoint PCR across a wide range of template
    • Robust yields with minimal optimization
    • Convenient product formats (stand-alone enzyme, master mixes, and Quick-Load® formats)
    • Hot start version allows room temperature reaction setup and does not require a separate activation step 
    • Compatible with standard Taq protocols

    Applications

    • High sensitivity PCR
    • High throughput PCR
    • Routine PCR
    • GC-rich PCR
    • AT-rich PCR
    • Primer extension
    • Colony PCR
    • Long PCR (up to ~6 kb genomic)

    PCR Selection Tool

    Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme technology and bring unparalleled confidence to your experiments.

    OneTaq Buffer Recommendations

    NEB recommends choosing your OneTaq Buffer based on amplicon % GC content.

    OneTaq vs Other Commercially Available Polymerases

    Comparison of OneTaq DNA Polymerase to Other Commercially Available Polymerases (non-hot start)
    Amplification of a selection of high GC human genomic DNA templates demonstrates OneTaq performance. PCR experiments included 30 amplification cycles. Purity (A) and Yield (B) were calculated via microfluidic analysis from triplicate reactions. Competitor polymerases were cycled according to manufacturer's recommendations.

    OneTaq 2X Master Mix with Standard Buffer

    Amplification of a selection of sequences with varying GC content from human and C. elegans genomic DNA using OneTaq 2X Master Mix with Standard Buffer. Amplicon sizes are indicated next to gel, and GC content is indicated above gel. Marker M is the 1 kb DNA Ladder (NEB# N3232).

    OneTaq 2X Master Mix with GC Buffer

    Amplification of a selection of sequences with varying GC content from human genomic DNA using OneTaq 2X Master Mix with GC Buffer. GC content is indicated above gel. Marker M is the 1 kb DNA Ladder (NEB# N3232).

    OneTaq Hot Start vs Other Commercially Available Hot Start Polymerases

    Comparison of OneTaq Hot Start DNA Polymerases to Other Commercially Available Hot Start Polymerases
    Reactions containing high GC human genomic DNA templates were set up at room temperature. PCR experiments included 30 cycles. Purity (A) and Yield (B) were calculated via microfluidic analysis from triplicate reactions. OneTaq polymerases were used with GC Buffer. Some OneTaq reactions also contained High GC Enhancer (striped bars). Competitor polymerases were cycled according to manufacturer's recommendations and included GC enhancers when supplied (striped bars).

    Recommended Time for Enzyme Activation

    Recommended time for enzyme activation of commercially available Hot Start

    DNA Polymerase Selection Chart

    NEB offers a guidelines for choosing the correct DNA polymerase for your application by providing a list of specific properites.
    Several factors govern which polymerase should be used in a given application, including: 

    Template/product specificity: Is RNA or DNA involved? Is the 3´ terminus at a gap, nick or at the end of the template? 

    Removal of existing nucleotides: Will the nucleotide(s) be removed from the existing polynucleotide chain as part of the protocol? If so, will they be removed from the 5´ or the 3´ end? 

    Thermal stability: Does the polymerase need to survive incubation at high temperature or is heat inactivation desirable? 

    Fidelity: Will subsequent sequence analysis or expression depend on the fidelity of the synthesized products?